Peptide Nucleic Acids

by Nielsen, Peter E.

• ISBN13: 9780896039766
• ISBN10: 0896039765
• Format: Hardcover
• Copyright: 2002-08-01
• Publisher: Humana Pr Inc

Summary

Peter Eigil Nielsen has assembled a critically evaluated collection of key PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process.

Table of Contents

Preface	v
Contributors	ix
I Introduction
PNA Technology	3	(26)
Peter E. Nielsen
II Chemistry
Solid Phase Synthesis of PNA Oligomers	29	(14)
Frederik Back
Synthesis of PNA-Peptide Conjugates	43	(10)
Satish Kumar Awasthi
Peter E. Nielsen
Parallel Synthesis of PNA-Peptide Conjugate Libraries	53	(6)
Satish Kumar Awasthi
Peter E. Nielsen
Thermodynamics of PNA Interactions with DNA and RNA	59	(32)
Tommi Ratilainen
Bengt Norden
III PNA Technology
Measurement of PNA Binding to Double-Stranded DNA	91	(20)
Thomas Bentin
Georg I. Hansen
Peter E. Nielsen
PNA-Mediated Immobilization of Supercoiled DNA	111	(8)
Thomas Bentin
Peter E. Nielsen
PNA Openers and Their Applications	119	(12)
Vadim V. Demidov
Maxim D. Frank-Kamenetskii
Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes	131	(14)
Miaomiao Wang
Bruce A. Armitage
IV Diagnostics
PNA-Mediated PCR Clamping: Applications and Methods	145	(20)
Deborah G. Murdock
Douglas C. Wallace
PNA Clamping Techniques for the Determination of Oncogene Mutations	165	(16)
Marcus Schuermann
Moira Behn
PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis	181	(14)
Brett Williams
Henrik Stender
James M. Coull
Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices	195	(16)
Gabor L. Igloi
V Antisense and Antigene
Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells	211	(14)
Dwaine A. Braasch
David R. Corey
Synthesis of Cell-Penetrating Peptide-PNA Constructs	225	(12)
Margus Pooga
Ursel Soomets
Tamas Bartfai
Ulo Langel
Antisense Inhibition of Bacterial Gene Expression and Cell Growth	237	(12)
Liam Good
In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops	249	(10)
Niels Erik Mollegaard
Peter E. Nielsen
VI Animal Studies
In Vitro and in Vivo Studies on Pharmacokinetics and Metabolism of PNA Constructs in Rodents	259	(12)
Edward Kristensen
Index	271