Phage Display of Peptides and Proteins : A Laboratory Manual

by Kay, Brian K.; Winter, Jill; McCafferty, John

• ISBN13: 9780124023802
• ISBN10: 0124023800
• Format: Paperback
• Copyright: 1996-08-01
• Publisher: Academic Pr

Summary

Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references

Table of Contents

Foreword

Biology of the Filamentous Bacteriophage

Principles and Applications of Phage Display

Vectros for Phage Display

Construction of Random Peptide Libraries in Bacteriophage

Construction and Screening of Antibody Display Libraries

Phagemeid-Displayed Peptide Libraries

Phage Libraries Displaying Random Peptides

Derived Form a Target Sequence

Display and Selection of Proteins on Genetic Packages

A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand

Interactions Made Possible By Linkage of Recogntion and Replication Functions

Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design

Use of Filamentous Virus Particles as Immunogens

Screening Phage Displayed Random Peptide Libraries

Phage Display Factors Affecting Planning Efficieny

Preparation of Second Generation Phage Libraries

Nonradioactive Sequencing of Random Peptide Recombinant Phage

Measurement of Peptide Binding Affinities Using Fluorescence Polarization

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