Pharmaceutical Biotechnology Concepts and Applications

by Walsh, Gary

• ISBN13: 9780470012444
• ISBN10: 0470012447
• Edition: 1st
• Format: Hardcover
• Copyright: 2007-08-13
• Publisher: WILEY

Summary

Pharmaceutical Biotechnology offers students taking Pharmacy and related Medical and Pharmaceutical courses a comprehensive introduction to the fast-moving area of biopharmaceuticals. With a particular focus on the subject taken from a pharmaceutical perspective, initial chapters offer a broad introduction to protein science and recombinant DNA technology- key areas that underpin the whole subject. Subsequent chapters focus upon the development, production and analysis of these substances. Finally the book moves on to explore the science, biotechnology and medical applications of specific biotech products categories. These include not only protein-based substances but also nucleic acid and cell-based products. introduces essential principles underlining modern biotechnology- recombinant DNA technology and protein science an invaluable introduction to this fast-moving subject aimed specifically at pharmacy and medical students includes specific 'product category chapters' focusing on the pharmaceutical, medical and therapeutic properties of numerous biopharmaceutical products. entire chapter devoted to the principles of genetic engineering and how these drugs are developed. includes numerous relevant case studies to enhance student understanding no prior knowledge of protein structure is assumed

Author Biography

Dr Gary Walsh: University of Limerick, Ireland

Table of Contents

Preface

Acronyms

Pharmaceuticals, biologics and biopharmaceuticals

Introduction to pharmaceutical products

Biopharmaceuticals and pharmaceutical biotechnology

History of the pharmaceutical industry

The age of biopharmaceuticals

Biopharmaceuticals: current status and future prospects

Protein structure

Introduction

Overview of protein structure

Higher level structure

Protein stability and folding

Protein post-translational modifi cation

Gene manipulation and recombinant DNA technology

Introduction

Nucleic acids: function and structure

Recombinant production of therapeutic proteins

Classical gene cloning and identifi cation

The drug development process

Introduction

Discovery of biopharmaceuticals

The impact of genomics and related technologies upon drug discovery

Gene chips

Proteomics

Structural genomics

Pharmacogenetics

Initial product characterization

Patenting

Delivery of biopharmaceuticals

Nasal, transmucosal and transdermal delivery systems

Preclinical studies

Pharmacokinetics and pharmacodynamics

Toxicity studies

The role and remit of regulatory authorities

Conclusion

Sources and upstream processing

Introduction

Sources of biopharmaceuticals

Upstream processing

Downstream processing

Introduction

Initial product recovery

Cell disruption

Removal of nucleic acid

Initial product concentration

Chromatographic purifi cation

High-performance liquid chromatography of proteins

Purifi cation of recombinant proteins

Final product formulation

Product analysis

Introduction

Protein-based contaminants

Removal of altered forms of the protein of interest from the product stream

Detection of protein-based product impurities

Immunological approaches to detection of contaminants

Endotoxin and other pyrogenic contaminants

The cytokines: The interferon family

Cytokines

Cytokine receptors

Cytokines as biopharmaceuticals

The interferons

Interferon biotechnology

Conclusion

Cytokines: Interleukins and tumour necrosis factor

Introduction

Interleukin-2

Interleukin-1

Interleukin-11

Tumour necrosis factors

Growth factors

Introduction

Haematopoietic growth factors

Growth factors and wound healing

Therapeutic hormones

Introduction

Insulin

Glucagon

Human growth hormone

The gonadotrophins

Medical and veterinary applications of gonadotrophins

Additional recombinant hormones now approved

Conclusion

Recombinant blood products and therapeutic enzymes

Introduction

Haemostasis

Anticoagulants

Thrombolytic agents

Enzymes of therapeutic value

Antibodies, vaccines and adjuvants

Introduction

Traditional polyclonal antibody preparations

Monoclonal antibodies

Vaccine technology

Adjuvant technology

Nucleic-acid- and cell-based therapeutics

Introduction

Gene therapy

Vectors used in gene therapy

Gene therapy and genetic disease

Gene therapy and cancer

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