A Photographic Atlas for the Microbiology Laboratory

by Leboffe, Michael J.; Pierce, Burton E.

• ISBN13: 9780895826565
• ISBN10: 0895826569
• Edition: 3rd
• Format: Loose-leaf
• Copyright: 2004-10-01
• Publisher: Morton Pub Co

Table of Contents

Bacterial Growth Patterns	1	(12)
Growth Patterns on Agar	1	(8)
Growth Patterns in Broth	9	(1)
Aerotolerance	9	(1)
Cultivation of Anaerobes	10	(3)
Isolation Techniques	13	(10)
Streak Plate Method of Isolation	13	(1)
Chocolate Agar	14	(1)
Deoxycholate Agar	14	(2)
Endo Agar	16	(1)
Eosin Methylene Blue Agar	16	(1)
Hektoen Enteric Agar	17	(1)
MacConkey Agar	18	(1)
Mannitol Salt Agar	18	(1)
Phenylethyl Alcohol Agar	19	(1)
Salmonella-Shigella Agar	20	(1)
Tellurite Glycine Agar	20	(1)
Thiosulfate Citrate Bile Salts Agar	21	(1)
Xylose Lysine Desoxycholate Agar	22	(1)
Microscopy	23	(4)
Types of Microscopy	23	(4)
Bacterial Cellular Morphology and Simple Stains	27	(8)
Simple Stains	27	(1)
Negative Stain	28	(1)
A Gallery of Bacterial Cell Diversity	28	(7)
Bacterial Cell Structures and Differential Stains	35	(10)
Gram Stain	35	(3)
Acid-fast Stains	38	(2)
Capsule Stain	40	(1)
Endospore Stain	41	(1)
Flagella Stain	42	(1)
Wet Mount and Hanging Drop Preparations	43	(1)
Miscellaneous Structures	44	(1)
Differential Media	45	(38)
API 20 E® for Identification of Enterobacteriaceae and Other Gram-Negative Rods	45	(1)
Bacitracin Susceptibility Test	46	(1)
β-Lactamase Test	47	(1)
Bile Esculin Test	47	(1)
Blood Agar	48	(1)
Casease Test	49	(1)
Catalase Test	50	(1)
Citrate Test	51	(1)
Coagulase Tests	52	(1)
BBL CRYSTAL™ Identification System---Enteric/Nonfermenter (E/NF)	53	(1)
Decarboxylation Test	54	(1)
DNase Test Agar	55	(1)
Enterotube® II	56	(1)
Fermentation Tests (Purple Broth and Phenol Red Broth)	57	(2)
Gelatinase Test	59	(1)
Indole Test (SIM Medium)	60	(1)
Kligler's Iron Agar	61	(1)
Lipase Test	62	(2)
Litmus Milk Medium	64	(1)
Lysine Iron Agar	65	(1)
Malonate Test	66	(1)
Methyl Red Test	67	(1)
Motility Agar	67	(1)
Nitrate Reduction Test	68	(2)
Novobiocin Susceptibility Test	70	(1)
ONPG (o-Nitrophenyl-β-D-galactopyranoside) Test	70	(1)
Optochin Susceptibility Test	71	(1)
Oxidase Test	72	(1)
Oxidation-Fermentation Test	73	(1)
Phenylalanine Deaminase Test	74	(1)
PYR (Pyrrolidonyl Arylamidase) Test	75	(1)
Starch Hydrolysis	75	(1)
Sulfur Reduction (SIM Medium)	76	(1)
SXT (Trimethoprim-Sulfamethoxazole) Susceptibility Test	77	(1)
Triple Sugar Iron Agar	78	(1)
Urease Test	79	(1)
Voges-Proskauer Test	80	(3)
Quantitative Techniques	83	(6)
Standard Plate Count (Viable) Count	83	(2)
Direct Count (Petroff-Hausser)	85	(1)
Plaque Assay for Determination of Phage Titer	86	(2)
Urine Streak (Semiquantitative Method)	88	(1)
Medical, Food, and Environmental Microbiology	89	(10)
Antimicrobial Susceptibility Test (Kirby-Bauer Method and E Test)	89	(2)
Colilert Method For Testing Drinking Water	91	(1)
Membrane Filter Technique for Total Coliform Determination	92	(1)
Multiple Tube Fermentation Method for Total Coliform Determination	93	(2)
Bioluminescence	95	(1)
Methylene Blue Reductase Test	95	(1)
Snyder Test	96	(1)
Nitrogen Cycle	96	(3)
Host Defenses	99	(6)
Differential Blood Cell Count	99	(2)
Other Immune Cells and Organs	101	(4)
Molecular Techniques	105	(8)
DNA Extraction	105	(1)
Electrophoresis	106	(1)
Polymerase Chain Reaction	107	(1)
DNA Sequencing	108	(1)
Ultraviolet Radiation Damage and Repair	109	(2)
Ames Test	111	(2)
Serology	113	(10)
Precipitation Reactions	113	(2)
Agglutination Reactions	115	(2)
Enzyme-Linked Immunosorbent Assay (ELISA)	117	(2)
Fluorescent Antibody (FA) Technique	119	(1)
Western Blot Technique	120	(3)
Viruses	123	(6)
Introduction to Viruses---T4 Virus	123	(1)
Human Immunodeficiency Virus (HIV)	124	(1)
Viral Cytopathic Effects in Cell Culture	125	(3)
Hemadsorption in Cell Culture	128	(1)
Bacterial Pathogens	129	(30)
Mycology	159	(16)
Survey of Fungi	159	(2)
Yeasts of Medical or Economic Importance	161	(2)
Monomorphic Molds	163	(8)
Zygomycetes	163	(3)
Dermatophyes	166	(2)
Other Monomorphic Molds	168	(3)
Dimorphic Molds	171	(4)
Parasitic Protozoans	175	(12)
Protozoan Survey	175	(1)
Protozoans of Clinical Importance	176	(11)
Amoeboid Protozoans Found in Clinical Specimens	176	(3)
Ciliate Protozoans Found in Clinical Specimens	179	(1)
Flagellate Protozoans Found in Clinical Specimens	179	(4)
Sporozoan Protozoans Found in Clinical Specimens	183	(2)
Miscellaneous Protozoans Found in Clinical Specimens	185	(2)
Parasitic Helminths	187	(14)
Trematode Parasites Found in Clinical Specimens	187	(4)
Cestode Parasites Found in Clinical Specimens	191	(4)
Nematode Parasites Found in Clinical Specimens	195	(6)
Appendix Biochemical Pathways	201	(8)
Oxidation of Glucose: Glycolysis, Entner-Doudoroff, and Pentose-Phosphate Pathways	201	(3)
Oxidation of Pyruvate: The Krebs Cycle and Fermentation	204	(5)
References for Further Reading	209	(2)
Index	211