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9780716714446

Physical Biochemistry Applications to Biochemistry and Molecular Biology

by Freifelder, David M.
  • ISBN13:

    9780716714446

  • ISBN10:

    0716714442

  • Edition: 2nd
  • Format: Paperback
  • Copyright: 1982-08-15
  • Publisher: W. H. Freeman

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Summary

Suitable for advanced undergraduate and graduate students in biochemistry, this book provides clear, concise, well-exampled descriptions of the physical methods that biochemists and molecular biologists use.

Table of Contents

Preface to the Second Edition vii
Preface to the First Edition ix
Characterization of Macromolecules
1(38)
Part One DIRECT OBSERVATION 39(78)
Light Microscopy
40(32)
Electron Microscopy
72(45)
Part Two GENERAL LABORATORY METHODS 117(98)
Measurement of pH
118(11)
Radioactive Labeling and Counting
129(40)
Autoradiography
169(24)
Membrane Filtration and Dialysis
193(22)
Part Three SEPARATION AND IDENTIFICATION OF MATERIALS 215(146)
Chromatography
216(60)
Electrophoresis
276(47)
Immunological Methods
323(38)
Part Four HYDRODYNAMIC METHODS 361(132)
Sedimentation
362(93)
Partial Specific Volume and the Diffusion Coefficient
455(15)
Viscosity
470(23)
Part Five SPECTROSCOPIC METHODS 493(160)
Absorption Spectroscopy
494(43)
Fluorescence Spectroscopy
537(36)
Optical Rotary Dispersion and Circular Dichroism
573(30)
Nuclear Magnetic Resonance and Electron Spin Resonance
603(50)
Part Six MISCELLANEOUS METHODS 653(70)
Ligand Binding
654(31)
Miscellaneous Methods
685(38)
Answers to Problems 723(30)
Index 753

Supplemental Materials

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