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9780470856031

Physical Biochemistry : Principles and Applications

by
  • ISBN13:

    9780470856031

  • ISBN10:

    0470856033

  • Edition: 2nd
  • Format: Paperback
  • Copyright: 2009-07-20
  • Publisher: Wiley
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Summary

The second edition of this successful textbook explains the basic principles behind the key techniques currently used in the modern biochemical laboratory and describes the pros and cons of each technique and compares one to another. It is non-mathematical, comprehensive and approachable for students who are not physical chemists. - A major update of this comprehensive, accessible introduction to physical biochemistry. - Includes two new chapters on proteomics and bioinformatics. - Introduces experimental approaches with a minimum of mathematics and numerous practical examples. - Provides a bibliography at the end of each chapter Written by an author with many years teaching and research experience, "Physical Biochemistry: Principles and Applications, Second Edition" will prove invaluable to students of biochemistry, biophysics, molecular and life sciences and food science.

Author Biography

DAVID SHEEHAN, University College Cork, Ireland.

Table of Contents

Preface
Acknowledgements
Introduction
Special chemical requirements of biomolecules
Factors affecting analyte structure and stability
pH effects
Temperature effects
Effects of solvent polarity
Buffering systems used in biochemistry
How does a buffer work?
Some common buffers
Additional components often used in buffers
Quantitation, units and data handling
Units used in this text
Quantitation of protein and biological activity
Objectives of this book Bibliography
Chromatography
Principles of chromatography
The partition coefficient
Phase systems used in biochemistry
Liquid chromatography
Gas chromatography
Performance parameters used in chromatography
Retention
Resolution
Physical basis of peak broadening
Plate height equation
Capacity factor
Peak symmetry
Significance of performance criteria in chromatography
Chromatography equipment
Outline of standard system used
Components of a chromatography system
Stationary phases used
Elution
Modes of chromatography
Ion exchange
Gel filtration
Reversed phase
Hydrophobic interaction
Affinity
Immobilised metal affinity chromatography
Hydroxyapatite
Open-column chromatography
Equipment used
Industrial-scale chromatography of proteins
High-performance liquid chromatography
Equipment used
Stationary phases in HPLC
Liquid phases in HPLC
Fast protein liquid chromatography
Equipment used
Comparison with HPLC
Perfusion chromatography
Theory of perfusion chromatography
The practice of perfusion chromatography
Membrane-based chromatography systems
Theoretical basis
Applications of membrane-based separations
Chromatography of a sample protein
Designing a purification protocol
Ion exchange chromatography of a sample protein:Glutathione S-transferases
HPLC of peptides from glutathione S-transferases Bibliography
Spectroscopic Techniques
The nature of light
A brief history of the theories of light
Wave-particle duality theory of light
The electromagnetic spectrum
The Electromagnetic Spectrum
Transitions in spectroscopy
Ultraviolet/visible absorption spectroscopy
Physical basis
Equipment used in absorption spectroscopy
Applications of absorption spectroscopy
Fluorescence spectroscopy
Physical basis of fluorescence and related phenomena
Measurement of fluorescence and chemiluminescence
External quenching of fluorescence
Uses of fluorescence in binding studies
Protein-folding studies
Resonance energy transfer
Applications of fluorescence in cell biology
Spectroscopic techniques using plane-polarised light
Polarised light
Chirality in biomolecules
Circular dichroism (CD)
Equipment used in CD
CD of biopolymers
Linear dichroism (LD)
LD of biomolecules
Plasmon resonance spectroscopy
Infrared spectroscopy
Physical basis of infrared spectroscopy
Equipment used in infrared spectroscopy
Uses of infrared spectroscopy in structure d
Table of Contents provided by Publisher. All Rights Reserved.

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