Plant Cell Culture A Practical Approach

by Dixon, Richard A.; Gonzales, Robert A.

• ISBN13: 9780199634026
• ISBN10: 0199634025
• Edition: 2nd
• Format: Paperback
• Copyright: 1995-02-09
• Publisher: IRL Press

Summary

Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad readership, that made the first edition so successful. Internationally recognized experts describe the techniques used for isolating and manipulating cell cultures, and the central importance in plant biotechnology. The book will be of major interest to researchers in plant sciences in general, and specifically to botany, plant physiology, and biotechnology students.

Table of Contents

Isolation and maintenance of callus and cell suspension cultures

Isolation, culture, and regeneration of protoplasts

Applications of protoplast technology

Fusion and selection of somatic hybrids

Transient gene expression and stable transformation

Studies with viruses

Selection of plant cells for desirable characteristics

Inhibitor resistance

Cold tolerance

In vitro selection for salt tolerance

In vitro selection for disease/toxin resistance

Plant regeneration via embryonic suspension cultures

Applied aspects of plant regeneration

Micropropagation

Virus-free plants

Artificial seeds

Cryopreservation

Secondary products from cultured cells and organs I: molecular and cellular approaches

Secondary products from cultured cells and organs II: large scale culture

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