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9781588299970

Plant Genomics

by ; ;
  • ISBN13:

    9781588299970

  • ISBN10:

    158829997X

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2009-04-28
  • Publisher: Humana Pr Inc

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Summary

Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools. In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and innovative, Plant Genomics: Methods and Protocols is an essential guide for all plant scientists who are interested in further studies in the area of genomics research.

Table of Contents

Prefacep. v
Contributorsp. ix
Role of Model Plant Speciesp. 1
New Technologies for Ultra-High Throughput Genotyping in Plantsp. 19
Genetic Maps and the Use of Syntenyp. 41
A Simple TAE-Based Method to Generate Large Insert BAC Libraries from Plant Speciesp. 57
Transcript Profiling and Expression Level Mappingp. 81
Methods for Functional Proteomic Analysesp. 93
Stable Transformation of Plantsp. 111
Transient Transformation of Plantsp. 131
Bridging the Gene-to-Function Knowledge Gap Through Functional Genomicsp. 153
Heterologous and Cell-Free Protein Expression Systemsp. 175
Functional Genomics and Structural Biology in the Definition of Gene Functionp. 199
In situ Analysis of Gene Expression in Plantsp. 229
Plant and Crop Databasesp. 243
Plant Genome Annotation Methodsp. 263
Molecular Plant Breeding: Methodology and Achievementsp. 283
Practical Delivery of Genes to the Marketplacep. 305
Ecological Genomics of Natural Plant Populations: The Israeli Perspectivep. 321
Genome Sequencing Approaches and Successesp. 345
Indexp. 359
Table of Contents provided by Ingram. All Rights Reserved.

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