Plant Genomics

by Somers, Daryl J.; Langridge, Peter; Gustafson, J. Perry

• ISBN13: 9781588299970
• ISBN10: 158829997X
• Edition: 1st
• Format: Hardcover
• Copyright: 2009-04-28
• Publisher: Humana Pr Inc

Summary

Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools. In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and innovative, Plant Genomics: Methods and Protocols is an essential guide for all plant scientists who are interested in further studies in the area of genomics research.

Table of Contents

Preface	p. v
Contributors	p. ix
Role of Model Plant Species	p. 1
New Technologies for Ultra-High Throughput Genotyping in Plants	p. 19
Genetic Maps and the Use of Synteny	p. 41
A Simple TAE-Based Method to Generate Large Insert BAC Libraries from Plant Species	p. 57
Transcript Profiling and Expression Level Mapping	p. 81
Methods for Functional Proteomic Analyses	p. 93
Stable Transformation of Plants	p. 111
Transient Transformation of Plants	p. 131
Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics	p. 153
Heterologous and Cell-Free Protein Expression Systems	p. 175
Functional Genomics and Structural Biology in the Definition of Gene Function	p. 199
In situ Analysis of Gene Expression in Plants	p. 229
Plant and Crop Databases	p. 243
Plant Genome Annotation Methods	p. 263
Molecular Plant Breeding: Methodology and Achievements	p. 283
Practical Delivery of Genes to the Marketplace	p. 305
Ecological Genomics of Natural Plant Populations: The Israeli Perspective	p. 321
Genome Sequencing Approaches and Successes	p. 345
Index	p. 359
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