Platelet Proteomics Principles, Analysis, and Applications

by Garcí; a-Alonso, Á; ngel; Senis, Yotis

• ISBN13: 9780470463376
• ISBN10: 0470463376
• Edition: 1st
• Format: Hardcover
• Copyright: 2011-05-31
• Publisher: Wiley

Summary

The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets.The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease. This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases.

Author Biography

Dr. ngel Garca is currently a Ramony y Cajal Research Fellow at the Pharmacology Department, Universidade de Santiago de Compostela, Spain. His main research focus is in the area of platelet proteomics, with special emphasis in cardiovascular research and platelet signating studies. He has written over twenty publications in peer-reviewed journals and has been an invited lecturer at the Platelets 2010 International Symposium (Jerusalem, Israel), and the XXII Congress of the International Society on Thombosis and Haemostasis, held in Boston, United States, in 2009, where he chaired a session on novel approaches to understanding platelet biology. Dr. Garcia was a postdoctoral research associate of the Proteomics Group at the Oxford Glycobiology Institute, University of Oxford (UK), from 2001 to 2005. Dr. Yotis Senis is currently a British Heart Foundation Research Fellow at the University of Birmingham, United Kingdom. He is a senior member of the Birmingham Platelet Group Dr. Senis research interests include the identification of novel platelet surface glycoproteins and determining their biological and molecular functions. He is also interested in the functional roles of protein tyrosine phosphatases in platelet and thrombosis. Dr. Senis has been an invited lecturer at the Platelet 2010 International Symposium (Jerusalem, Israel) and the Gordon Research Conference on the Cell Biology of Megakaryocytes and Platelets (Galyeston, Texas). He has written over twenty publication on platelet signaling and thrombosis.

Table of Contents

Contributors	p. ix
Foreword	p. xiii
Preface	p. xv
Acronyms	p. xvii
General Overview: Platelets, Sample Preparation, and Mass Spectrometry-Based Proteomics
Platelets and Their Role in Thrombotic and Cardiovascular Disease: The Impact of Proteomic Analysis	p. 3
Mass-Sepectrometry-Based Proteomics: General Overview and Posttranslational Modification Analysis in the Context of Platelet Research	p. 27
Sample Preparation Variable in Platelet Proteomics for Biomarker Research	p. 67
Analysis of the Platelet Proteome: Global Approaches and Subproteomes
Two-Dimensional Gel Electrophoresis: Basic Principles and Application to Platelet Signaling Studies
The Platelet Membrane Proteome	p. 111
Proteomics of Platelet Granules, Organelles, and Releasate	p. 139
The Platelet Microparticle Proteome	p. 159
N-Terminal Combined Fractional Diagonal Chromatographic (COFRADIC) Analysis of the Human Platelet Protome	p. 185
Integrated ôOMICSö and Application to Disease
Serial Analysis of Gene Expression (SAGE) For Studying the Platelet and Megakaryocyte Transcriptome	p. 209
The Application of Microarray Analysis and Its Integration with Proteomics for Study of Platelet-Associated Disorders	p. 231
Platelet Functional Genomics	p. 253
Systems Biology to Study Platelet-Related Bleeding Disorders	p. 285
Platelet Proteomics in Transfusion Medicine	p. 321
Cardiovascular Proteomics	p. 341
Index	p. 381
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