Platelets and Megakaryocytes

by Gibbins, Jonathan M.; Mahaut-Smith, Martyn P.

• ISBN13: 9781588290113
• ISBN10: 1588290115
• Format: Hardcover
• Copyright: 2004-08-01
• Publisher: Humana Pr Inc

Summary

For both volumes:A comprehensive collection of cutting-edge methods for the molecular and cellular analysis of platelets and megakaryocytes. Volume 1 details basic and advanced techniques for analyzing platelet and megakaryocyte function. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. The techniques for study of megakaryocyte function allow the measurement of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. Volume 2 offers techniques for studying many aspects of signaling in platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts, and the measurement of intracellular calcium levels. There are also techniques for study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. This volume also describes the application of molecular and post-genomic methods are to the study of platelets and megakaryocytes. All protocols follow the successful Methods in Molecular Biology''žÂ¢ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The two volumes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Table of Contents

Preface	v
Contents of Volume I	xiii
Contributors	xv
Color Plates	xix
Part I. Perspectives
Signaling Receptors on Platelets and Megakaryocytes	3	(30)
Donna Woulfe
Jing Yang
Nicolas Prevost
Peter O'Brien
Ryan Fortna
Massimiliano Tognolini
Hong Jiang
Jie Wu
Lawrence F. Brass
Manipulation of Gene Expression in Megakaryocytes	33	(24)
Nikla R. Emambokus
George J. Murphy
Jonathan Frampton
In Vitro Changes of Platelet Parameters: Lessons From Blood Banking	57	(16)
Jose Rivera
Maria L. Lozano
Vicente Vicente
Pharmacological Approaches to Studying Platelet Function: An Overview	73	(14)
Susanna M. O. Hourani
Inhibitors of Cellular Signaling Targets: Designs and Limitations	87	(18)
Chris J. Vlahos
Louis F. Stancato
Part II Signaling Techniques
Ligand-Binding Assays for Collagen	105	(10)
Stephanie M. Jung
Masaaki Moroi
Use of Radiolabeled 2-Methylthio-ADP to Study P2Y Receptors on Platelets and Cell Lines	115	(10)
Pierre Savi
Jean-Marc Herbert
Ligand-Binding Assays: Fibrinogen	125	(14)
Dermot Cox
Techniques for the Analysis of Proteins by SDS-Polyacrylamide Gel Electrophoresis and Western Blotting	139	(14)
Jonathan M. Gibbins
Study of Tyrosine Kinases and Protein Tyrosine Phosphorylation	153	(16)
Jonathan M. Gibbins
Protein Tyrosine Phosphatases	169	(10)
Matthew L. Jones
Alastair W. Poole
The Study of Serine-Threonine Kinases	179	(22)
Gertie Gorter
Jan Willem Akkerman
Phosphoinositides: Lipid Kinases and Phosphatases	201	(12)
Bernard Payrastre
Isolation and Analysis of Platelet Lipid Rafts	213	(16)
Corie N. Shrimpton
Karine Gousset
Fern Tablin
Jose A. Lopez
Measurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell Cultures	229	(22)
Philippe Ohlmann
Beatrice Hechler
Jean-Pierre Cazenave
Christian Gachet
Measurement and Manipulation of Intracellular Ca2+ in Single Platelets and Megakaryocytes	251	(26)
Michael J. Mason
Martyn P. Mahaut-Smith
Patch-Clamp Recordings of Electrophysiological Events in the Platelet and Megakaryocyte	277	(24)
Martyn P. Mahaut-Smith
Evaluation of Nitrotyrosine-Containing Proteins in Blood Platelets	301	(12)
Khalid M. Naseem
Rocio Riba
Max Troxler
Nitric Oxide Signaling in Platelets	313	(22)
Sylvia Y. Low
K. Richard Bruckdorfer
Snake Venom Toxins Affecting Platelet Function	335	(14)
Robert K. Andrews
Elizabeth E. Gardiner
Michael C. Berndt
Peptide Synthesis in the Study of Collagen-Platelet Interactions	349	(16)
C. Graham Knight
Catherine M. Onley
Richard W. Farndale
Platelet Permeabilization	365	(16)
Robert Flaumenhaft
Part III. Molecular and Post-Genomic Techniques
Using Retroviruses to Express Genes in Primary Megakaryocyte Lineage Cells	381	(16)
Meenakshi Gaur
George J. Murphy
Jonathan Frampton
Andrew D. Leavitt
Use of Antisense Oligonucleotide Technology to Investigate Signaling Pathways in Megakaryocytes	397	(10)
Hava Avraham
Shalom Avraham
Radoslaw Zagozdzon
GFP Fusion Proteins to Study Signaling in Live Cells	407	(14)
Simon A. Walker
Gyles E. Cozier
Peter J. Cullen
Two-Dimensional Polyacrylamide Gel Electrophoresis for Platelet Proteomics	421	(14)
Katrin Marcus
Helmut E. Meyer
Preparation of mRNA and cDNA Libraries From Platelets and Megakaryocytes	435	(20)
Benjamin Z. S. Paul
Jiango Jin
Satya P. Kunapuli
Platelet Receptor Structures and Polymorphisms	455	(24)
Thomas J. Kunicki
Steven Head
Daniel R. Salomon
Gene Array Technology and the Study of Platelets and Megakaryocytes	479	(12)
Lloyd T. Lam
Emery H. Bresnick
Index	491