A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery

by Chen; Taosheng

• ISBN13: 9781420070507
• ISBN10: 1420070509
• Edition: 1st
• Format: Hardcover
• Copyright: 2009-12-21
• Publisher: CRC Press

Summary

The development of suitable assays, the integration of appropriate technology, and the effective management of the essential infrastructure are all critical to the success of any high-throughput screening (HTS) endeavor. However, few scientists have the multidisciplinary experience needed to control all aspects of an HTS drug discovery project. A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery integrates the experience of diverse experts who offer fundamental and practical guidance across numerous situations.The book first discusses assay developments for important target classes such as protein kinases and phosphatases, proteases, nuclear receptors, G protein-coupled receptors, ion channels, and heat shock proteins. It next examines assay developments for cell viability, apoptosis, and infectious diseases. The contributors explore the application of emerging technologies and systems, including image-based high content screening, RNA interference, and primary cells. Finally, they discuss the essential components of the integrated HTS process, such as screening automation, compound library management, the screening of natural products from botanical sources, and screening informatics.Designed to motivate researchers to bring further advances to the field, this volume provides practical guidance on how to initiate, validate, optimize, and manage a bioassay intended to screen large collections of compounds. Drawing on the knowledge from experts actively involved in assay development and HTS, this is a resource that is both comprehensive and focused.

Table of Contents

Preface	p. vii
Acknowledgments	p. ix
About the Editor	p. xi
Contributors	p. xiii
Assay Development for Protein Kinases and Phosphatases	p. 1
Fluorescence-Based Biochemical Protease Assay Formats	p. 25
Assay Development for Nuclear Hormone Receptors	p. 49
Assay Development for G Protein-Coupled Receptors and Ion Channels	p. 59
Assay Development for Heat Shock Proteins	p. 83
Assay Development for Cell Viability and Apoptosis for High-Throughput Screening	p. 99
Assay Development for Antimicrobial Drug Discovery	p. 123
Image-Based High-Content Screening	p. 143
Application of RNA Interference in Drug Discovery	p. 157
Assay Development Using Primary and Primary-Like Cells	p. 169
Screening Automation	p. 183
Compound Library Management	p. 193
Unique Discovery Aspects of Utilizing Botanical Sources	p. 213
Screening Informatics	p. 233
Index	p. 265
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