A Practical Guide to Basic Laboratory Andrology

by Lars Björndahl , David Mortimer , Christopher L. R. Barratt , Jose Antonio Castilla , Roelof Menkveld , Ulrik Kvist , Juan G. Alvarez , Trine B. Haugen

• ISBN13: 9780521735902
• ISBN10: 0521735904
• Edition: 1st
• Format: Spiral Bound
• Copyright: 2010-05-24
• Publisher: Cambridge University Press

Summary

This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.

Author Biography

Lars Bjrndahl is Consultant, Center for Andrology and Sexual Medicine, Clinic of Endocrinology, Department of Medicine, Karolinska University Hospital and Karolinska Institutet, Huddinge, Stockholm, Sweden. David Mortimer is President and Principal Consultant of Oozoa Biomedical Vancouver, British Columbia, Canada. Christopher L.R. Barratt is Professor of Reproductive Medicine, University of Dundee, Scotland. Jose Antonio Castilla is Director of Andrology and Embryology Laboratory, Hospital Virgen de las Nieves, Spain, and Medical Director of Sperm Bank CEIFER, Granada, Spain. Roelof Menkveld is Director of the Andrology Laboratory, Department of Obstetrics and Gynaecology, Tygerberg Academic Hospital and University of Stellenbosch, Tygerberg, South Africa. Ulrik Kvist is Associate Professor of Andrology, Director of Andrology Laboratory, Center for Andrology and Sexual Medicine, Clinic of Endocrinology, Department of Medicine Huddinge, Stockholm, Karolinska University Hospital and Karolinska Institutet, Sweden. Juan G. Alvarez is Scientific Director, Institut Marques, Barcelona, Spain. Trine B. Haugen is Professor, Faculty of Health Sciences, Oslo University College, Oslo, Norway.

Table of Contents

List of authors	p. vi
List of abbreviations	p. vii
Introduction	p. 1
Basic physiology	p. 5
Basic semen analysis	p. 33
Extended semen analysis	p. 77
Sperm function tests	p. 113
Tests of sperm-cervical mucus interaction	p. 147
Sperm preparation	p. 167
Sperm cryobanking	p. 189
Preparation of surgically retrieved spermatozoa	p. 219
Quality management and accreditation	p. 227
Risk management	p. 249
Reproductive toxicology	p. 257
Appendices
Reference values	p. 261
Equipment required for a basic andrology laboratory	p. 277
Supply companies	p. 287
Recommendations on the application of CASA technology in the analysis of spermatozoa	p. 291
Home testing	p. 295
Example laboratory and report forms	p. 297
Comparison of replicate counts	p. 305
Safety	p. 313
Index	p. 323
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