Practical Skills in Biomolecular Sciences

by Reed, Rob; Holmes, David; Weyers, Jonathan; Jones, Allan

• ISBN13: 9780132391153
• ISBN10: 0132391155
• Format: Paperback
• Copyright: 2007-07-05
• Publisher: Prentice Hall

Summary

Practical Skills in Biomolecular Science, is an indispensable that provides useful support at all stages of a degree course and underpins any practical course in biochemistry, biomedical science, genetics, immunology and microbiology. Sections key transferable skills, including chapters on time management, working with others, note taking, revising, assessment and exams, and preparing a cv. Chapters on bioinformatics on the preparation and use of calibration curves. Up-dated material on the use of the Internet and world wide web. Coverage of numeracy and statistics to provide support and guidance in this tricky area. Each chapter has study exercises to reinforce learning with problems and practical exercises. Answers are given at the back of the book for all exercises. Each chapter is supported by a section giving printed and electronic sources for further study. Worked examples and "how to" boxes set out the essential procedures in a step-by-step manner. Key points highlight critical features of methodology. Use of margin tips, definitions and illustrations. Use of two-colour text throughout the book.

Table of Contents

List of boxes

Preface

Guided tour

For the student

Acknowledgements

List of abbreviations

Study and examination skills

The importance of transferable skills

Managing your time

Working with others

Taking notes from lectures and texts

Learning and revising

Curriculum options, assessments and exams

Preparing your curriculum vitae

Information technology and library resources

Finding and citing published information

Evaluating information

Using online resources

Bioinformatics - Internet resources

Using spreadsheets

Word processors, databases and other packages

Communicating information

Organising a poster display

Giving a spoken presentation

General aspects of scientific writing

Writing essays

Reporting practical and project work

Writing literature surveys and reviews

Fundamental laboratory techniques

Your approach to practical work

Health and safety

Working with liquids

Basic laboratory procedures

Principles of solution chemistry

pH and buffer solutions

Introduction to microscopy

Setting up and using a light microscope

The investigative approach

Making and recording measurements

SI units and their use

Scientific method and design of experiments

Project workHandling cells and tissues

Sterile technique and microbial culture

Isolating, identifying and naming microbes

Working with animal and plant tissues and cells

Culture systems and growth measurement

Homogenisation and fractionation of cells and tissues

Analytical techniques

Calibration and its application to quantitative analysis

Immunological methods

Radioactive isotopes and their uses

Light measurement

Basic spectroscopy

Advanced spectroscopy and spectrometry

Centrifugation

Chromatography - separation methods

Chromatography - detection and analysis

Principles and practice of electrophoresis

Advanced electrophoretic techniques

Electroanalytical techniques

Assaying biomolecules and studying metabolism

Analysis of biomolecules: fundamental principles

Assaying amino acids, peptides and proteins

Assaying lipids

Assaying carbohydrates

Assaying nucleic acids and nucleotides

Protein purification

Enzyme studies

Membrane transport processes

Photosynthesis and respiration

Genetics

Mendelian genetics

Bacterial and phage genetics

Molecular genetics I - fundamental principles

Molecular genetics II - PCR and related applications

Molecular genetics III - genetic engineering techniques

Analysis and presentation of data

Using graphs

Presenting data in tables

Hints for solving numerical problems

Descriptive statistics

Choosing and using statistical tests

Answers to study exercises

Index

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