Preparation & Assay of Enzymes, Supplement to Volumes 1 and 2

by Kaplan; Colowick

• ISBN13: 9780121818050
• ISBN10: 0121818055
• Format: Hardcover
• Copyright: 1961-12-28
• Publisher: Elsevier Science

Summary

The critically acclaimed laboratory standard, Methods in Enzymologylt;/b>, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Table of Contents

Preface	v
Contributors to Volume V	vii
Outline of Volumes I, II, III, IV, V, VI	xiii
Errata for Volumes I, II, III, VI	xxiv
Section I. General Preparative Procedures
Column Chromatography of Proteins: Substituted Celluloses	3	(24)
Elbert A. Peterson
Herbert A. Sober
Column Chromatography of Proteins: Calcium Phosphate	27	(6)
Osten Levin
Preparative Electrophoresis	33	(18)
M. Bier
Preparation and Solubilization of Particles: Bacterial	51	(10)
Arnold F. Brodie
Preparation of Microsomes and Submicrosomal Fractions: Mammalian	61	(8)
Philip Siekevitz
Preparation and Solubilization of Particles: Higher Plant	69	(4)
George C. Webster
Preparation and Solubilization of Particles: Chromatophores	73	(4)
J. W. Newton
The Technique of Mammalian Cell Culture	77	(13)
Leon Levintow
Harry Eagle
Quantitative Colonial Growth of Isolated Mammalian Cells	90	(29)
Richard G. Ham
Theodore T. Puck
Techniques for Study of Mammalian Cell Cultures: Extraction of Enzymes	119	(3)
Irving Lieberman
James L. Gingold
Preparation and Use of Protoplasts	122	(15)
John Spizizen
Section II. Enzymes of Carbohydrate Metabolism
Lysozymes from Rabbit Spleen and Dog Spleen	137	(4)
Pierre Jolles
Amylomaltase	141	(4)
Herbert Wiesmeyer
Glycogen Synthetase from Rat Liver	145	(3)
Luis F. Leloir
Sara H. Goldemberg
Cyclodextrin Transglycosylase (Bacillus macerans Amylase)	148	(7)
Dexter French
Hyaluronic Acid-Synthesizing System from Group A Streptococcus	155	(4)
Alvin Markovitz
Albert Dorfman
Glucuronide-Forming Enzymes	159	(12)
G. J. Dutton
I. D. E. Storey
Trehalose and Sucrose-Forming Enzymes
UDPG-glucose-6-phosphate Transglucosylase from Yeast	165	(2)
Luis F. Leloir
Enrico Cabib
UDPG-fructose Transglucosylase from Wheat Germ	167	(3)
Luis F. Leloir
Carlos E. Cardini
UDPG-fructose-6-phosphate Transglucosylase	170	(1)
Luis F. Leloir
Carlos E. Cardini
GDPM Pyrophosphorylase	171	(3)
Agnete Munch-Petersen
Enzymes of the Leloir Pathway	174	(16)
Elizabeth S. Maxwell
Kiyoshi Kurahashi
Herman M. Kalckar
Enzymes of Glucuronic and Galacturonic Acid Metabolism in Bacteria	190	(18)
Gilbert Ashwell
D-Xylulokinase from Calf Liver	208	(4)
Gilbert Ashwell
β-Galactosidase (Crystalline)	212	(7)
K. Wallenfels
Mutarotase	219	(7)
Ronald Bentley
Hexokinase from Baker's Yeast	226	(10)
Robert A. Darrow
Sidney P. Colowick
Phosphoglyceric Acid Mutase	236	(7)
S. Grisolia
Glycerate 2,3-Diphosphatase	243	(4)
S. Grisolia
D-Xylulose 5-Phosphate-3-epimerase	247	(6)
Jerard Hurwitz
L-Ribulose 5-Phosphate-4-epimerase	253	(5)
B. L. Horecker
Phosphoribulokinase	258	(3)
Jerard Hurwitz
D-Xylulose 5-Phosphate Phosphoketolase	261	(5)
B. L. Horecker
Ribulose Diphosphate Carboxylase from Spinach Leaves	266	(4)
E. Racker
Sedoheptulose-1,7-diphosphatase from Yeast	270	(2)
E. Racker
Fructose-1,6-diphosphatase from Spinach Leaves	272	(4)
E. Racker
Fructose-6-phosphate Phosphoketolase from Acetobacter xylinum	276	(4)
E. Racker
D-Xylulose-5-phosphate-3-Epimerase from Muscle	280	(3)
E. Racker
Phosphoketotetrose Aldolase from Rat Liver	283	(4)
Frixos C. Charalampous
Gluconic Dehydrogenase from Pseudomonas fluorescens	287	(4)
W. A. Wood
R. A. Fetting
B. C. Hertlein
2-Ketogluconokinase from Aerobacter aerogenes	291	(4)
W. A. Wood
E. W. Frampton
2-Keto-6-phosphogluconate Reductase from Pseudomonas fluorescens	295	(3)
W. A. Wood
E. W. Frampton
L-Ribulokinase	298	(4)
W. A. Wood
F. J. Simpson
Ribo-Deoxyribokinases	302	(8)
Bernard W. Agranoff
Ann Ginsburg
Bovine Liver Aldolase	310	(7)
R. J. Peanasky
Henry A. Lardy
Xylitol Dehydrogenases (DPN and TPN)	317	(6)
O. Touster
G. Montesi
Galactitol Dehydrogenase	323	(3)
D. R. D. Shaw
Inositol Dehydrogenase from Aerobacter aerogenes	326	(3)
Joseph Larner
Inositol-Cleaving Enzyme from Rat Kidney	329	(4)
Frixos C. Charalampous
Aldose Reductase	333	(2)
H. G. Hers
Mannose Isomerase of Pseudomonas saccharophila	335	(4)
Michael Doudoroff
D-Galactose Dehydrogenase of Pseudomonas saccharophila	339	(3)
Michael Doudoroff
L-Arabinose Dehydrogenase of Pseudomonas saccharophila	342	(2)
Michael Doudoroff
L-Arabinose Isomerase	344	(3)
Pauline Z. Smyrniotis
Xylose Isomerase	347	(3)
Milton W. Slein
D-Erythrose Isomerase	350	(2)
K. Uehara
Glycerate Kinase	352	(2)
Simon Black
Glycerokinase	354	(8)
Clark Bublitz
Otto Wieland
Triokinase	362	(3)
H. G. Hers
Pyruvate Kinase (Fluorokinase)	365	(4)
Alisa Tietz
Severo Ochoa
Muscle Phosphorylase b	369	(4)
Edmond H. Fischer
Edwin G. Krebs
Phosphorylase b Kinase from Rabbit Skeletal Muscle	373	(4)
Edwin G. Krebs
Edmond H. Fischer
Enzymes Concerned with Interconversion of Liver Phosphorylases	377	(14)
T. W. Rall
Earl W. Sutherland
N-Acetylneuraminic Acid Aldolase	391	(3)
Donald G. Comb
Saul Roseman
3-Deoxy-D-arabino-heptulosonic Acid 7-Phosphate Synthetase from Escherichia coli	394	(4)
David B. Sprinson
P. R. Srinivasan
Masayuki Katagiri
5-Dehydroquinate Synthetase	398	(4)
David B. Sprinson
P. R. Srinivasan
Joel Rothschild
Lignin Formation	402	(6)
Walter J. Schubert
Glucosamine 6-Phosphate Formation from Glutamine (Rat Liver)	408	(6)
Burton M. Pogell
L-Glutamine-D-Fructose 6-Phosphate Transamidase from Escherichia coli	414	(4)
Sudhamoy Ghosh
Saul Roseman
Glucosamine 6-Phosphate Deaminase from Pig Kidney	418	(4)
Luis F. Leloir
Carlos E. Cardini
Glucosamine 6-Phosphate Deaminase from Escherichia coli	422	(4)
Donald G. Comb
Saul Roseman
Lactic Acid Racemase	426	(6)
Don Dennis
α-L-Glycerophosphate Dehydrogenase from Pig Brain	432	(11)
Robert L. Ringler
Thomas P. Singer
Section III. Enzymes of Lipid Metabolism
Fatty Acid Synthesis from Malonyl CoA	443	(8)
F. Lynen
Enzymes of Branched-Chain Amino Acid Metabolism	451	(10)
Minor J. Coon
William G. Robinson
Assay and Preparation of Yeast Aceto-CoA-Kinase	461	(6)
Paul Berg
Activating Enzymes for Higher Fatty Acids	467	(6)
William P. Jencks
Activation and Conjugation of Bile Acids	473	(3)
W. H. Elliott
Glycerokinase	476	(4)
Eugene P. Kennedy
Phosphorylcholine-Cytidyl Transferase	480	(2)
Eugene P. Kennedy
Phosphatidic Acid Phosphatase	482	(2)
Eugene P. Kennedy
Phosphorylcholine-Glyceride Transferase	484	(2)
Eugene P. Kennedy
Phosphorylcholine-Ceramide Transferase	486	(3)
Eugene P. Kennedy
Enzymes in Sterol Biogenesis	489	(10)
T. T. Tchen
Cortisone 5β Reductase from Rat Liver	499	(4)
Gordon M. Tomkins
Enzymes of Steroid Hydroxylation	503	(9)
Mika Hayano
Ralph I. Dorfman
Hydroxysteroid Dehydrogenases	512	(15)
Paul Talalay
δ5-3-Ketosteroid Isomerase from Pseudomonas testosteroni	527	(6)
Frank S. Kawahara
Steroid Ring A Δ-Dehydrogenases	533	(6)
H. Richard Levy
Lipoxidase	539	(3)
A. L. Tappel
Lipoprotein Lipase (Clearing Factor)	542	(4)
Edward D. Korn
Acyl Dehydrogenases from Pig and Beef Liver and Beef Heart	546	(11)
Helmut Beinert
β-Hydroxybutyryl CoA Racemase	557	(3)
Joseph R. Stern
Choline Kinase from Brewer's Yeast	560	(2)
Jonathan B. Wittenberg
Choline Dehydrogenase from Rat Liver	562	(8)
Tokuji Kimura
Thomas P. Singer
Propionyl CoA Carboxylase from Pig Heart	570	(6)
Alisa Tietz
Severo Ochoa
Mitochondrial Propionyl Carboxylase	576	(5)
M. Daniel Lane
Donald R. Halenz
Methylmalonyl CoA Isomerase	581	(6)
William S. Beck
Acrylyl Coenzyme A Aminase	587	(6)
P. Roy Vagelos
Section IV. Enzymes of Citric Acid Cycle
Itaconic Acid Enzymes	593	(4)
Ronald Bentley
Succinic Dehydrogenase	597	(17)
Paul Bernath
Thomas P. Singer
Aconitic Hydrase from Aspergillus niger	614	(3)
Nora E. Neilson
Conversion of P-Pyruvate to Oxalacetate (Plant)	617	(5)
Birgit Vennesland
Citratase and Isocitratase	622	(11)
H. H. Daron
I. C. Gunsalus
Malate Synthetase from Baker's Yeast	633	(4)
G. H. Dixon
H. L. Kornberg
Oxalate Decarboxylation	637	(4)
William B. Jakoby
Citrate-Cleavage Enzyme	641	(4)
Paul A. Srere
Isocitric Dehydrogenase (TPN-Linked) from Pig Heart (Revised Procedure)	645	(6)
G. W. E. Plaut
Formation and Breakdown of Acyl Lipoic Acids	651	(8)
Ernest Knight, Jr.
I. C. Gunsalus
Section V. Enzymes of Protein Metabolism
Collagenase from Clostridium histolyticum	659	(6)
Sam Seifter
Paul M. Gallop
Pancreatic Elastase	665	(8)
Ines Mandl
L-Alanine Dehydrogenase from Bacillus subtilis	673	(4)
J. M. Wiame
A. Pierard
F. Ramos
Glutamic Aspartic Transaminase from Pig Ventricles	677	(8)
Irwin W. Sizer
W. Terry Jenkins
Transaminases in Animal Tissue Homogenates	685	(13)
E. V. Rowsell
β-Alanine-α-alanine Transaminase (Pseudomonas)	698	(5)
Osamu Hayaishi
Yasutomi Nishizuka
Amino Acid-Activating Enzymes: Methods of Assay	703	(5)
M. P. Stulberg
G. D. Novelli
Bacterial Amino Acyl RNA Synthetases	708	(10)
Fred H. Bergmann
Tryptophan-Activating Enzyme	718	(4)
Earl W. Davie
Tyrosine-Activating Enzyme from Hog Pancreas	722	(4)
Richard S. Schweet
Amino Acid-Activating Enzymes from Guinea Pig Liver	726	(5)
Richard S. Schweet
Amino Acid Incorporation Enzymes	731	(5)
Mirko Beljanski
Thiazolidinecarboxylate Dehydrogenase	736	(2)
Cosmo G. Mackenzie
Sarcosine Dehydrogenase	738	(5)
Cosmo G. Mackenzie
Dale D. Hoskins
Dimethylthetin-Homocysteine Methylpherase	743	(5)
G. L. Cantoni
Catechol-O-methyl Transferase from Rat Liver	748	(4)
Julius Axelrod
S-Adenosyl-L-homocysteine-Cleaving Enzyme	752	(4)
Gabriel De La Haba
Adenosylmethionine Decarboxylase	756	(5)
Celia White Tabor
Propylamine Transferase (Spermidine Synthesis)	761	(4)
Celia White Tabor
Enzymes of γ-Aminobutyrate Metabolism (Bacterial)	765	(13)
William B. Jakoby
γ-Hydroxybutyrate Dehydrogenase from Clostridium aminobutyricum	778	(6)
John K. Hardman
Histidine Degradation (Formiminoglutamic Acid Metabolism)	784	(6)
Herbert Tabor
N5-Formyltetrahydrofolic Acid Glutamic Acid Transformylase from Hog Liver	790	(4)
Milton Silverman
Enzymes Involved in the Biosynthesis of Tryptophan	794	(13)
Oliver H. Smith
Charles Yanofsky
Kynurenine Hydroxylase	807	(2)
Osamu Hayaishi
Phenylalanine Hydroxylase	809	(7)
Seymour Kaufman
Aryl-4-Hydroxylase	816	(3)
Chozo Mitoma
Sidney Udenfriend
Tryptophan Hydroxylase	819	(1)
Chozo Mitoma
Herbert Weissbach
Conversion of Aspartic Acid to Homoserine	820	(7)
Simon Black
β-Methylaspartase from Clostridium tetanomorphum	827	(6)
H. A. Barker
R. D. Smyth
H. J. Bright
L. L. Ingraham
Transglutaminase	833	(5)
Heinrich Waelsch
Mary J. Mycek
Serine Hydroxymethylase	838	(5)
K. G. Scrimgeour
F. M. Huennekeens
Transamidinase	843	(5)
S. Ratner
Diaminopimelic Acid Synthesis in Escherichia coli	848	(3)
Charles Gilvarg
N-Succinyl-L-diaminopimelic Deacylase	851	(2)
S. H. Kindler
N-Succinyl-L-diaminopimelic Glutamic Transaminase	853	(5)
B. Peterkofsky
Diaminopimelic Racemase	858	(6)
Elizabeth Work
Diaminopimelic Decarboxylase	864	(6)
Elizabeth Work
Proline Reductase	870	(5)
Thressa C. Stadtman
Proline Racemase	875	(3)
Thressa C. Stadtman
Δ-Piperideine-2-carboxylate and Δ-Pyrroline-2-carboxylate Reductase	878	(5)
Alton Meister
Δ-Aminolevulinic Acid Dehydrase from Rhodopseudomonas spheroides	883	(2)
David Shemin
Porphyrin Synthesis	885	(11)
L. Bogorad
Enzymes of Isovaleryl CoA Metabolism	896	(7)
Minor J. Coon
Carbamyl Phosphate Synthesis and Utilization	903	(22)
Mary Ellen Jones
Aspartate Transcarbamylase from Escherichia coli	925	(6)
Margaret Shepherdson
Arthur B. Pardee
Threonine and Allothreonine Aldolase	931	(5)
David M. Greenberg
Cystathionine and Homoserine Cleavage	936	(6)
David M. Greenberg
Serine and Threonine Dehydratases	942	(9)
David M. Greenberg
Threonine Synthetase from Neurospora	951	(8)
Martin Flavin
Pyrroline-5-carboxylate Reductase	959	(5)
David M. Greenberg
Sulfate-Activating Enzymes	964	(13)
Phillips W. Robbins
Sulfate-Transferring Enzymes	977	(6)
John D. Gregory
Sulfite Cytochrome c Reductase	983	(4)
W. Farkas
R. W. Macleod
I. Fridovich
P. Handler
Mercaptopyruvate: Cyanide Transsulfurase	987	(3)
J. W. Hylin
β-Mercaptopyruvate: Sulfite Transsulfurase	990	(2)
Bo H. Sorbo
Reduction of Sulfoxide and Disulfides	992	(7)
Simon Black
Author Index	999	(20)
Subject Index	1019