Principles of Fluorescence Spectroscopy

by Lakowicz, Joseph R.

• ISBN13: 9780387312781
• ISBN10: 0387312781
• eBook ISBN(s): 9780387463124
• Additional ISBN(s): 9780306460937
• Edition: 3rd
• Format: Hardcover
• Copyright: 2006-08-04
• Publisher: Springer Nature
• More Book Details
• Purchase Benefits

Summary

The third edition of the established classic text reference, Principles of Fluorescence Spectroscopy, will enhance upon the earlier editions' successes. Organized as a textbook for the learning student or the researcher needing to acquire the core competencies, Principles of Fluorescence Spectroscopy, 3e will maintain the emphasis on basics, while updating the examples to include recent results from the literature. The third edition also includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. This full-color textbook features the following: - Problem sets following every chapter - Glossaries of commonly used acronyms and mathematical symbols - Appendices containing a list of recommended books which expand on various specialized topics - Sections describing advanced topics will indicate as such, to allow these sections to be skipped in an introductory course, allowing the text to be used for classes of different levels Principles of Fluorescence Spectroscopy, 3 rd edition, is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Author Biography

Dr. Joseph R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine, Baltimore, and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the author of the widely used text, Principles of Fluorescence Spectroscopy now in its 3rd edition.

Table of Contents

Introduction to Fluorescence

Instrumentation for Fluorecence Spectroscopy

Fluorophores

Time-Domain Lifetime Measurements

Frequency-Domain Lifetime Measurements

Solvent and Environmental Effects

Dynamics of Solvent and Spectral Relaxation

Quenching of Fluorescence

Mechanisms and Dynamics of Fluorescence Quenching

Fluorescence Anisotropy

Time-Dependent Anisotropy Decays

Advanced Anisotropy Concepts

Energy Transfer

Time Resolved Energy Transfer and Conformational Distributions of Biopolymers

Energy Transfer to Multiple Acceptors, in One-, Two- or Three-Dimensions

Protein Fluorescence

Time-Resolved Protein Fluorescence

Multiphoton Excitation and Microscopy

Fluorescence Sensing

Novel Fluorophobes

DNA Technology

Fluorescence Lifetime Imaging Microscopy

Single-Molecule Detection

Fluorescence Correlation Spectroscopy

Radiative Decay Engineering: Metal-Enhanced Fluorescence

Radiative Decay Engineering: Surface Plasmon-Coupled Emission

Corrected Emission Spectra

Fluorescent Lifetime Standards

Additional Reading

Answers to Problems

Index

Table of Contents provided by Publisher. All Rights Reserved.