Protein Engineering A Practical Approach

by Rees, Anthony R.; Sternberg, Michael J. E.; Wetzel, Ronald

• ISBN13: 9780199631384
• ISBN10: 0199631387
• Format: Paperback
• Copyright: 1993-02-04
• Publisher: IRL Press

Summary

Protein engineering is the rational modification or redesign of proteins using genetic engineering. Thus, it is now possible to modify enzyme specifics, remodel antibodies, and redesign many multi-domain proteins for therapeutic purposes. While the procedures for the introduction of mutations have become routine, predicting and understanding the effects of these mutations can be complicated. This volume provides a comprehensive guide to the methods used at every stage of the engineering process, from the choice of mutation strategy, through protein stability studies, to critical evaluations of mammalian, yeast, and bacterial host expression systems. Protein Engineering: A Practical Approach is the first practical guide to this fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry. It combines a thorough theoretical foundation with detailed protocols and will be invaluable to all research workers in the area, from graduate students to senior investigators.

Table of Contents

Methods of Structural Analysis of Proteins -

Protein Crystallography

Methods of Structural Analysis of Proteins

Nuclear Magnetic Resonance

Molecular Sequence Data- Bases and Their Uses

Design of Protein Structures

From Protein Sequence to Structure

The Use of Theoretical Methods in Protein Engineering

Principles of protein stability - part 1 - reversible unfolding of proteins: kinetic and thermodynamic analysis

Principles of protein stability - part 2 - enhanced folding and stabilization of proteins by suppression of aggregation in vitro and in vivo

Principles of protein turnover: possible manipulations

Chemical approaches to expression of proteins

Protein engineering of antibody combining sites

Selection of variants of antibodies and other protein molecules using display on the surface of bacteriophage fd

Expression of proteins in prokaryotic systems - principles and 'case' studies

Expression of proteins in yeast

Genetic engineering of the processing and secretory pathway of an inflammatory cytokine in mammalian cells: a case study

Industrial applications of protein engineering and scale-up of protein production: l-antitrypsin, a case study

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