Protein Phosphatase Protocols

by Ludlow, John W.

• ISBN13: 9780896034686
• ISBN10: 0896034682
• Format: Hardcover
• Copyright: 1998-06-01
• Publisher: Humana Pr Inc

Summary

Cutting-edge techniques presented with foolproof, step-by-step instructions make this collection indispensable for investigating the structure and function of protein serine/threonine phosphatases. The book contains methods designed to enable the investigator to isolate, identify, and assay phosphatase activities from both prokaryotic and eukaryotic sources, and includes a discussion of the functional significance of this group of enzymes with respect to cell growth and development. Written by leaders with extensive practice in their use, each method is time-tested and sufficiently detailed to ensure reproducibility for both new and established investigators.

Table of Contents

Preface	v	(6)
Contributors	xi
1 Prokaryotic Protein-Serine/Threonine Phosphatases	1	(22)
Peter J. Kennelly
2 Protein Phosphatase Type 1 and Type 2A Assays	23	(12)
S. Derek Killilea
Qi Cheng
Zhi-Xin Wang
3 Analyzing Gene Expression with the Use of Serine/Threonine Phosphatase Inhibitors	35	(6)
Axel H. Shonthal
4 Inhibitor-1, a Regulator of Protein Phosphatase 1 Function	41	(18)
John H. Connor
Hai Quan
Carey Oliver
Shirish Shenolikar
I(1)(PP2A) and I(2)(PP2A): Two Potent Protein Phosphatase 2A-Specific Inhibitor Proteins	59	(8)
Mei Li
Zahi Damuni
6 Control of PP1 Activity Through Phosphorylation by Cyclin-Dependent Kinases	67	(12)
Norbert Berndt
7 Regulation of Neuronal PP1 and PP2A During Development	79	(24)
Elizabeth Collins
Alistair T.R. Sim
8 PTPA Regulating PP2A as a Dual Specificity Phosphatase	103	(14)
Veerle Janssens
Christine Van Hoof
Wilfried Merlevede
Jozef Goris
9 Microinjection and Immunological Methods in the Analysis of Type 1 and 2A Protein Phosphatases from Mammalian Cells	117	(20)
Patric Turowski
Ned J.C. Lamb
10 Use of Immunocomplexed Substrate for Detecting PP1 Activity	137	(8)
John W. Ludlow
Deirdre A. Nelson
Nancy A. Krucher
11 The Biochemical Identification and Characterization of New Species of Protein Phosphatase 1	145	(12)
Monique Beullens
Willy Stalmans
Mathieu Bollen
12 The Relationship Between Insulin Signaling and Protein Phosphatase 1 Activation	157	(12)
Louis Ragolia
Najma Begum
13 Analysis of the Isoforms of Protein Phosphatase 1 (PP1) Isoforms with Polyclonal Peptide Antibodies	169	(16)
Margherita Tognarini
Emma Villa-Moruzzi
14 Expression of Mouse Protein Phosphatase 2C in Eschericia coli and COS 7 Cells	185	(6)
Takayasu Kobayashi
Kazuyuki Kusuda
Motoko Ohnishi
Naoki Chida
Shinri Tamura
15 Expression of Functional Protein Phosphatase 1 Catalytic Subunit in E. coli	191	(10)
Mariam Dohadwala
Norbert Berndt
16 Protein Phosphatase 2A and Protein Phosphatase X Genes in Arabidopsis thaliana	201	(12)
Gemma Pujol
Albert Ferrer
Joaquin Arino
17 Separation of Protein Phosphatase Type 2C Isozymes by Chromatography on Blue Sepharose	213	(6)
Susanne Klumpp
Dagmar Selke
18 Chromatographic Isolation of PP2A from Limulus Lateral Eyes: Conventional and Small Scale Methods	219	(16)
Samuel C. Edwards
Travis B. Van Dyke
Timothy H. Van Dyke
David L. Brautigan
19 Purification and Assay of the Ptc/Tpd1 Protein Phosphatase 2C from the Yeast Saccharomyces cerevisiae	235	(8)
Matthew K. Robinson
Eric M. Phizicky
20 Molecular Cloning of Protein Phosphatase Type 2C Isoforms from Retinal cDNA	243	(8)
Dagmar Selke
Susanne Klumpp
Benjamin Kaupp
Arnd Baumann
21 Analysis of Protein Interactions Between Protein Phosphatase 1 and Noncatalytic Subunits Using the Yeast Two-Hybrid Assay	251	(12)
Nadja T. Ramaswamy
Brian K. Dalley
John F. Cannon
22 Identifying Protein Phosphatase 2A Interacting Proteins Using the Yeast Two-Hybrid Method	263	(16)
Brent McCright
David M. Virshup
23 Protein Phosphatase 2A Regulatory Subunits: cDNA Cloning and Analysis of mRNA Expression	279	(14)
Julie A. Zaucha
Ryan S. Westphal
Brian E. Wadzinski
24 Synthetic Lethal Screening in Protein Phosphatase Pathways	293	(12)
Jianhong Zheng
John F. Cannon
25 The Search for the Biological Function of Novel Yeast Ser/Thr Phosphatases	305	(10)
Joaquin Arino
Francesc Posas
Josep Clotet
Index	315