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JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.
Preface | |
Contributors | |
Introduction | |
Introduction to Protein Purification | |
Chromatography | |
Introduction to Chromatography | |
Gel Filtration | |
Ion Exchange Chromatography | |
High-Resolution Reversed-Phase Chromatography | |
Hydrophobic Interaction Chromatography | |
Immobilized Metal Ion Affinity Chromatography | |
Covalent Chromatpgraphy | |
Affinity Chromatography | |
Affinity Ligands from Chemical Combinatorial Libraries | |
Affinity Ligands from Biological Combinatorial Libraries | |
Other Separation Methods and Related Techniques | |
Membrane Separations | |
Refolding of Inclusion Body Proteins from E. coli | |
Purification of PEGylated Proteins | |
Electrophoresis | |
Electrophoresis in Gels | |
Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients | |
Two-Dimensional Electrophoresis in Proteomics | |
Protein Elution and Blotting Techniques | |
Capillary Electrophoretic Separations | |
Separation Method Optimization | |
High Throughput Screening Techniques on Protein Purification | |
Index | |
Table of Contents provided by Publisher. All Rights Reserved. |
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