Protein Sensors of Reactive Oxygen Species, Part A: Selenoproteins and Thioredoxin

by Packer; Sies

• ISBN13: 9780121822484
• ISBN10: 0121822486
• Edition: 1st
• Format: Hardcover
• Copyright: 2002-03-01
• Publisher: Elsevier Science

Summary

This volume of Methods in Enzymology is concerned with the rapidly developing field of selenoprotein synthesis and its related molecular genetics. Progressive information on the topics of proteins as redox sensors, selenoproteins, and the thioredoxin system is studied using methods such as bioinformatics, DNA chip technology, cell biology, molecular genetics, and enzymology. The information on novel selenoproteins identified from genomic sequence data, as well as current knowledge on glutathione peroxidases, selenoprotein P, iodothyronine deiodinases, and thioredoxin reductases, is presented in a method-based approach.

Table of Contents

Contributors to Volume 347	ix
Preface	xv
Volume in Series	xvii
Section I. Selenoproteins
Selenoprotein Biosynthesis: Purification and Assay of Components Involved in Selenocysteine Biosynthesis and Insertion in Escherichia coli	3	(14)
Martin Thanbichler
August Bock
Selenocysteine Insertion Sequence Element Characterization and Selenoprotein Expression	17	(7)
Marla J. Berry
Glover W. Martin III
Roza Tujebajeva
Elisabeth Grundner-Culemann
John B. Mansell
Nadya Morozova
John W. Harney
Transfer RNAs That Insert Selenocysteine	24	(16)
Bradley A. Carlson
Dolph L. Hatfield
Purification and Analysis of Selenocysteine Insertion Sequence-Binding Protein 2	40	(9)
Paul R. Copeland
Donna M. Driscoll
Nonsense-Mediated Decay: Assaying for Effects on Selenoprotein mRNAs	49	(8)
Xiaolei Sun
Lynne E. Maquat
Novel Selenoproteins Identified from Genomic Sequence Data	57	(13)
Alain Lescure
Daniel Gautheret
Alain Krol
Semisynthesis of Proteins Containing Selenocysteine	70	(14)
Robert J. Hondal
Ronald T. Raines
Mammalian Selenoprotein Gene Signature: Identification and Functional Analysis of Selenoprotein Genes Using Bioinformatics Methods	84	(17)
Gregory V. Kryukov
Vadim N. Gladyshev
Estimation of Individual Types of Glutathione Peroxides	101	(12)
Regina Brigelius-Flohe
Kirtsten Wingler
Cordula Muller
High-Throughput 96-Well Microplate Assays for Determining Specific Activities of Glutathione Peroxidase and Thioredoxin Reductase	113	(8)
Allen D. Smith
Orville A. Levander
Selenoprotein P	121	(4)
Gavin E. Arteel
Lars-Oliver Klotz
Darius P. Buchczyk
Helmut Sies
Iodothyronine Deiodinases	125	(43)
Josef Kohrle
Expression and Regulation of Thioredoxin Reductases and Other Selenoproteins in Bone	168	(11)
Franz Jakob
Katja Becker
Ellen Paar
Regina Ebert-Duemig
Norbert Schutze
Selenoprotein W	179	(8)
Philip D. Whanger
Genetic and Functional Analysis of Mammalian Sep 15 Selenoprotein	187	(11)
Easwari Kumaraswamy
Konstantin V. Korotkov
Alan M. Diamond
Vadim N. Gladyshev
Dolph L. Hatfield
Selenocysteine Lyase from Mouse Liver	198	(5)
Hisaaki Mihara
Nobuyoshi Esaki
Selenocysteine Methyltransferase	203	(5)
Bernhard Neuhierl
August Bock
Phospholipid-Hydroperoxide Glutathione Peroxidase in Sperm	208	(5)
Antonella Roveri
Leopold Flohe
Matilde Maiorino
Fulvio Ursini
In Vivo Antioxidant Role of Glutathione Peroxidase: Evidence from Knockout Mice	213	(13)
Xin Gen Lei
Recombinant Expression of Mammalian Selenocysteine-Containing Thioredoxin Reductase and Other Selenoproteins in Escherichia coli	226	(10)
Elias S. J. Arner
Mammalian Thioredoxin Reductases as Hydroperoxide Reductases	236	(8)
Liangwei Zhong
Arne Holmgren
Tryparedoxin and Tryparedoxin Peroxidase	244	(15)
Leopold Flohe
Peter Steinert
Hans-Jurgen Hecht
Birgit Hofmann
Trypanothione and Tryparedoxin in Ribonucleotide Reduction	259	(8)
R. Luise Krauth-Siegel
Heide Schmidt
Selenium- and Vitamin E-Dependent Gene Expression in Rats: Analysis of Differentially Expressed mRNAs	267	(12)
Alexandra Fischer
Josef Pallauf
Gerald Rimbach
Section II. Thioredoxin
Overview	279	(7)
Hiroshi Masutani
Junji Yodoi
Thioredoxin and Glutaredoxin Isoforms	286	(11)
Alexios Vlamis-Gardikas
Arne Holmgren
Mammalian Thioredoxin Reductases	297	(10)
Takashi Tamura
Thressa C. Stadtman
Mitochondrial Thioredoxin Reductase and Thiol Status	307	(10)
Alberto Bindoli
Maria pia Rigobello
Protein Electrophoretic Mobility Shift Assay to Monitor Redox State of Thioredoxin in Cells	317	(10)
Neil A. Bersani
Jason R. Merwin
Nathan I. Lopez
George D. Pearson
Gary F. Merrill
Recycling of Vitamin C by Mammalian Thioredoxin Reductase	327	(5)
James M. MAY
Thioredoxin Cytokine Action	332	(7)
Yumiko Nishinaka
Hajime Nakamura
Junji Yodoi
Identification of Thioredoxin-Linked Proteins by Fluorescence Labeling Combined with Isoelectric Focusing/Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis	339	(10)
Joshua H. Wong
Hiroyuki Yano
Young-Moo Lee
Myeong-Je Cho
Bob B. Buchanan
Thioredoxin and Mechanism of Inflammatory Response	349	(11)
Takashi Okamoto
Kaori Asamitsu
Toshifumi Tetsuka
Redox State of Cytoplasmic Thioredoxin	360
Daniel Ritz
Jon Beckwith
Thioredoxin, Thioredoxin Reductase, and Thioredoxin Peroxidase of Malaria Parasite Plasmodium falciparum	37o	(382)
Stefan M. Kanzok
Stefan Rahles
Katja Becker
R. Heiner Schirmer
Human Placenta Thioredoxin Reductase: Preparation and Inhibitor Studies	382	(12)
Stephen Gromer
Heiko Merkle
R. Heiner Schirmer
Katja Becker
Classification of Plant Thioredoxins by Sequence Similarity and Intron Position	394	(9)
Yves Meyer
Florence Vignols
Jean Philippe Reichheld
Ferredoxin-Dependent Thioredoxin Reductase: A Unique Iron-Sulfur Protein	403	(9)
Peter Schurmann
Plant Thioredoxin Gene Expression: Control by Light, Circadian Clock, and Heavy Metals	412	(9)
Stephane D. Lemaire
Myroslawa Miginiac-Maslow
Jean-Pierre Jacquot
Thioredoxin Genes in Lens: Regulation by Oxidative Stress	421	(15)
Kailash C. Bhuyan
Pabbathi G. Reddy
Durga K. Bhuyan
Thioredoxin Overexpression in Transgenic Mice	436	(5)
Hajime Nakamura
Akira Mitsui
Junji Yodoi
Multiplex Reverse Transcription-Polymerase Chain Reaction for Determining Transcriptional Regulation of Thioredoxin and Glutaredoxin Pathways	441	(10)
Carmen Pueyo
Juan Jurado
Maria-Jose Prieto-Alamo
Fernando Monje-Casas
Juan Lopez-Barea
Redox Regulation of Cell Signaling by Thioredoxin Reductases	451	(12)
Qi-An Sun
Vadim N. Gladyshev
Author Index	463	(34)
Subject Index	497