Protein Sequencing Protocols

by Smith, Bryan John

• ISBN13: 9780896039759
• ISBN10: 0896039757
• Edition: 2nd
• Format: Hardcover
• Copyright: 2002-11-01
• Publisher: Humana Pr Inc

Summary

Bryan John Smith updates his much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to analysis of protein sequence information via the powerful new tools of bioinformatics. Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides for both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination.

Table of Contents

Preface	v
Contributors	xi
Strategies for Handling Polypeptides on a Micro-Scale	1	(18)
Bryan John Smith
Paul Tempst
SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing	19	(12)
Bryan John Smith
Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization	31	(10)
Michael J. Dunn
Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization	41	(8)
Michael J. Dunn
Enzymatic Cleavage of Proteins	49	(14)
Bryan John Smith
Chemical Cleavage of Polypeptides	63	(20)
Bryan John Smith
Enzymatic Preparation and Isolation of Glycopeptides	83	(8)
David J. Harvey
Analytical and Micropreparative Capillary Electrophoresis of Peptides	91	(10)
Alan J. Smith
High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues	101	(10)
Christoph W. Turck
Hydrolysis of Samples for Amino Acid Analysis	111	(12)
Ian Davidson
Amino Acid Analysis: Precolumn Derivatization Methods	123	(10)
G. Brent Irvine
Ian Davidson
Post Column Amino Acid Analysis	133	(10)
Alan J. Smith
Amino Acid Analysis Using Pre-Column Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: Analysis of Hydrolyzed Proteins and Electroblotted Samples	143	(12)
Steven A. Cohen
Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection	155	(14)
Petr Jandik
Jun Cheng
Nebojsa Avdalovic
D-Amino Acid Analysis	169	(12)
Andrea Scaloni
Maurizio Simmaco
Francesco Bossa
Validation of Amino Acid Analysis Methods	181	(14)
Andrew J. Reason
Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins	195	(16)
Fiona M. Greer
Howard R. Morris
Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry	211	(10)
Darryl J. C. Pappin
Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes	221	(14)
Anna Shevchenko
Shamil Sunyaev
Adam Liska
Peer Bork
Andrej Shevchenko
Direct Identification of Proteins in Ultracomplex Mixtures: Applications to Proteome Analysis	235	(12)
David M. Schieltz
John R. Yates, III
Identification of PTH-Amino Acids by HPLC	247	(22)
Gregory A. Grant
Mark W. Crankshaw
Protein Sequencer Maintenance and Troubleshooting	269	(18)
Bryan Dunbar
N-Terminal Protein Sequencing for Special Applications	287	(14)
Philip J. Jackson
Identification of Phosphorylation Sites by Edman Degradation	301	(8)
Laurey Steinke
Richard G. Cook
Validation of Protein Sequencing in a Regulated Laboratory	309	(10)
Philip J. Jackson
Neil Dodsworth
Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method	319	(14)
David R. Dupont
Sylvia W. Yuen
Kenneth S. Graham
Chemical Modifications of Proteins as an Aid to Sequence Analysis	333	(22)
Alex F. Carne
Deblocking of N-Terminally Modified Proteins	355	(10)
Hisashi Hirano
Roza M. Kamp
Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid	365	(6)
Jacek Mozdzanowski
Identification of Sites of Glycosylation	371	(14)
David J. Harvey
Analysis of Sites of Protein Phosphorylation	385	(14)
Alastair Aitken
Michele Learmonth
Quantitation and Location of Disulfide Bonds in Proteins	399	(12)
Alastair Aitken
Michele Learmonth
Getting the Most from Your Protein Sequence	411	(20)
Richard R. Copley
Robert B. Russell
Appendix 1: Letter Codes, Structures, Masses, and Derivatives of Amino Acids	431	(34)
James P. Turner
Appendix 2: Protein Consensus Sequence Motifs	465	(22)
Alastair Aitken
Index	487