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9780199636181

Protein Structure A Practical Approach

by Creighton, T. E.
  • ISBN13:

    9780199636181

  • ISBN10:

    0199636184

  • Additional ISBN(s):

    9780199630011

  • Edition: 2nd
  • Format: Paperback
  • Copyright: 1997-05-08
  • Publisher: IRL Press

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Summary

Protein Structure: A Practical Approach gives general methods for determining protein structure that will be very widely applicable, from the simple electrophoretic, chromatographic, and spectrophotometric techniques to the relatively more complex and sophisticated methods of mass spectrometry and ultracentrifugation. This second edition has been fully updated to embrace the most recent developments in the field, and will be invaluable to all involved in protein research.

Table of Contents

Protein molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis
Protein analysis by mass spectrometry
Immunological detection of proteins of known sequence
Identification of common post-translational modifications
Peptide mapping
Counting integral numbers of residues by chemical modification
Disulfide bonds between cysteine residues
Analysis of protein conformation by gel electrophoresis
Hydrodynamic properties of proteins
How to determine the molar absorbance coefficient of a protein
Optical spectroscopy to characterize protein confirmation and conformational changes
Measuring the conformational stability of a protein
Immunochemical analysis of protein conformation
Stabilization of protein structure by solvents
Table of Contents provided by Publisher. All Rights Reserved.

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