Protein Structure and Protein Function A Practical Approach 2 Volume Set

by Creighton, Thomas E.

• ISBN13: 9780199636174
• ISBN10: 0199636176
• Additional ISBN(s): 9780199630646
• Edition: 2nd
• Format: Hardcover
• Copyright: 1997-07-24
• Publisher: Oxford University Press

Summary

Protein Structure, 2nd ed.


1. Protein molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis


2. Protein analysis by mass spectrometry


3. Immunological detection of proteins of k

Table of Contents

Protein Structure, 2nd ed.

Protein molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis

Protein analysis by mass spectrometry

Immunological detection of proteins of known sequence

Identification of common post-translational modifications

Peptide mapping

Counting integral numbers of residues by chemical modification

Disulfide bonds between cysteine residues

Analysis of protein conformation by gel electrophoresis

Hydrodynamic properties of proteins

How to determine the molar absorbance coefficient of a protein

Optical spectroscopy to characterize protein confirmation and conformational changes

Measuring the conformational stability of a protein

Immunochemical analysis of protein conformation

Stabilization of protein structure by solvents Protein Function, 2nd ed.

Identifying proteins by two-dimensional gel; electrophoresis

Expressing cloned genes in Escherichia coli

Folding proteins

Ligand binding

Ligand blotting

Affinity labelling

Chemical cross-linking and protein function

Analysis of sequence-specific DNA-binding proteins

Purification of sequence-specific DNA-binding proteins by affinity chromatography

Chemical modification

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