Protein-Ligand Interactions A Practical Approach 2 Volume-Set

by Harding, Stephen E.; Chowdhry, Babur Z.

• ISBN13: 9780199637485
• ISBN10: 0199637482
• Format: Paperback
• Copyright: 2001-03-22
• Publisher: Oxford University Press

Summary

A ligand is an atom, molecule or ion that can bind to a specific site on a protein and the interactions between any protein and its ligands are fundamental and essential for the protein to function properly. The first volume covers the principal hydrodynamic and calorimetric techniques for studying protein-ligand interactions. The second volume covers all the principal spectroscopic and structural methods for investigating protein-ligand interactions.

Table of Contents

Hydrodynamics and Calorimetry List of protocols Abbreviations

Protein-ligand interactions and their analysis

Equilibrium dialysis and rate dialysis

Quantitative characterization of ligand binding by chromatography

Sedimentation velocity analytical ultracentrifugation

Sedimentation equilibrium in the analytical ultracentrifuge

Surface plasmon resonance

Capillary electrophoresis

Molecular electro-optics

High-flux X-ray and neutron solution scattering

Isothermal titration calorimetry of biomolecules

Differential scanning microcalorimetry

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Index Structure and Spectroscopy

List of protocols Abbreviations

X-ray crystallographic studies of protein-ligand interactions

Molecular modelling

Circular dichroism

Generic techniques for fluorescence measurements of protein-ligand interactionsreal-time kinetics and spatial imaging

Stopped-flow techniques

Protein-ligand interactions studied by FTIR spectroscopy: methodological aspects

Protein-ligand interactions studied by Raman and resonance Raman spectroscopy

Electrospray ionization mass spectrometry

Nitroxide spin labels as paramagnetic probes

NMR studies of protein-ligand interactions

Quantification and mapping of protein-ligand interactions at the single molecule level by atomic force microscopy

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Index

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