Proteins and Proteomics: A Laboratory Manual

by Simpson, Richard J.

• ISBN13: 9780879695545
• ISBN10: 0879695544
• Edition: 1st
• Format: Paperback
• Copyright: 2003-01-01
• Publisher: Cold Spring Harbor Laboratory Pr

Summary

Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. Proteins and Proteomics: A Laboratory Manual,authored by Richard Simpson of the Ludwig Institute for Cancer Research,Melbourne, provides the first authoritative and wide-ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, Proteins and Proteomicsprovides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists, developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an excellent resource for protein chemists and biochemists.

Table of Contents

Proteins and Proteomics Companion Web Site	vii
Preface	ix
Acknowledgments	xi
Foreword	xiii
Introduction to Proteomics	1	(38)
One-dimensional Polyacrylamide Gel Electrophoresis	39	(52)
Preparation of Cellular and Subcellular Extracts	91	(52)
Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients	143	(76)
Reversed-phase High-performance Liquid Chromatography	219	(68)
Amino- and Carboxy-terminal Sequence Analysis	287	(56)
Peptide Mapping and Sequence Analysis of Gel-resolved Proteins	343	(82)
The Use of Mass Spectrometry in Proteomics	425	(172)
Proteomic Methods for Phosphorylation Site Mapping	597	(72)
Characterization of Protein Complexes	669	(126)
Making Sense of Proteomics: Using Bioinformatics to Discover a Protein's Structure, Functions, and Interactions	795	(114)
APPENDICES
Reference Tables	837	(8)
Techniques	845	(52)
Cautions	897	(10)
Suppliers	907	(2)
Index	909