Proteomic Profiling and Analytical Chemistry

by Ciborowski; Silberring

• ISBN13: 9780444593788
• ISBN10: 0444593780
• eBook ISBN(s): 9780444593788, 9780444593917
• Format: Paperback
• Copyright: 2013-02-28
• Publisher: Elsevier Science
• More Book Details
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Summary

This book is designed to help scientists without a strong background in analytical chemistry to understand basic analytical principles so they can apply them to proteomics profiling. It will also help researchers with an analytical chemistry background to break into the proteomics field. The title focuses on practical applications for proteomic research so readers are guided to design better experiments and to more easily interpret the resulting data. * filling gap between many specialized courses and book in mass spectrometry, proteomics and analytical chemistry. * real life examples illustrating importance of problems in quantitation and validation of biomarkers from perspective of analytical chemistry with reference to sources. Problems for further discussion at the end of eachc chapter. * help in designing and executing proteomic experiments with sound analytics.

Table of Contents

Introduction

Biomolecules

Fundamental Strategies of Protein and Peptide Sample Preparation

Protein Extraction and Precipitation

Immunoaffinity Depletion of High-Abundant Proteins for Proteomic Sample

Gel Electrophoresis

Quantitative Measurements in Proteomics

Proteomic Database Search and Analytical Quantification

Microarrays and Dynamics of Fluorescent Dyes

Design and Statistical Analysis of Mass-Spectrometry Based Quantitative Proteomics Data

Principles of Analytical Validation

Validation in Proteomics and Regulatory Affairs

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