Protocols in Molecular Neurobiology

by Longstaff, Alan; Revest, Patricia

• ISBN13: 9780896031999
• ISBN10: 0896031993
• Format: Paperback
• Copyright: 1992-10-01
• Publisher: Humana Pr Inc

Summary

Each chapter identifies a problem in neurobiology, showing how the solution of that problem will advance knowledge in the field. The approaches taken to solve the problem are clearly explained, providing sufficient detail for users to readily reproduce the technique and its results. In keeping with the goal of the series, each technique is designed to work, even the first time it is used.

Table of Contents

Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis

The Isolation of Genomic DNA from Invertebrates

Analysis of Mitochondrial DNA Mutations

The Design and Use of Oligonucleotides

The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related DNA Sequences

Isolation of Photoreceptor Cell-Specific MEKA cDNA by Differential Hybridization

Generation of Isoform-Specific Antisera from Cloned cDNAs: Application to Multiple Forms of the Na/K-ATPase Expressed in Rat Brain

Determination of Transcriptional Initiation Sites and Their Usage in the Nervous System

Mapping of Trans-Acting Factor Binding in the Nervous System

In Situ Hybridization to Brain Tissue Sections Using Labeled Single-Strand Complementary RNA Probes

In Situ Hybridization Histochemistry Using Alkaline Phosphatase- Labeled Oligodeoxynucleotide Probe

The Identification of Neuropeptide Gene Regulatory Elements in Transgenic Mice

Expression of Exogenous Ion Channels and Neurotransmitter Receptors in RNA- Injected Xenopus Oocytes

Analysis of Insulin and Insulin-Like Growth Factor-I Receptors in Neural Tissues

Use of Affinity Chromatography in Purification of A1 Adenosine Receptors from Rat Brain Membranes

Purification and Structure of L-Type Calcium Channels

Purification and Reconstitution of the Ryanodine-Sensitive Ca2+ Release Channel Complex from Muscle Sarcoplasmic Reticulum

Identification of a Ligand-Gated Ion Channel by Photoaffinity Labeling and Microsequencing

Voltage-Gated Ion Channels: Electrophysiological Approaches

An Electrophysiological Approach to the Regulation of Neuronal Voltage-Activated Calcium Channels by Guanine Nucleotide Binding Proteins

Index

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