Protocols for in Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

by Jain, S. Mohan; Saxena, Praveen K.

• ISBN13: 9781603272865
• ISBN10: 1603272860
• Edition: 1st
• Format: Hardcover
• Copyright: 2009-05-12
• Publisher: Humana Pr Inc

Summary

This book provides a detailed step-by-step description of protocols for the establishment of in vitro cultures of important medicinal plants, their mass multiplication in controlled environment, and step-wise secondary metabolite analysis. In addition, many of these protocols will provide a basis for much needed efforts in germplasm conservation of medicinal species at the brink of extinction. Medicinal plant species are diverse and distinct in their growth patterns and their yield must be defines it terms of chemistry and in vitro cultures provide the vehicle to accomplish this objective. A comprehensive compilation of technical details and protocols for in vitro culture of medicinal plants is not currently available. The proposed is expected to be well received in academia, industry, and Government agencies.

Table of Contents

Preface	p. v
Contributors	p. xi
Micropropagation and Chemical Analysis
Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives	p. 3
In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium, Assessment of Genetic Fidelity of Micropropagated Plants, and Simultaneous Quantitation of Reserpine, Ajmaline, and Ajmalicine	p. 17
Medicinal Properties, In Vitro Protocols and Secondary Metabolite Analyses of Scots Pine	p. 35
Saussurea medusa Cell Suspension Cultures for Flavonoid Production	p. 53
Large-scale In Vitro Multiplication of Crataeva nurvala	p. 61
Bilberry In Vitro Protocols and Analyses of Phenolic Compounds	p. 71
In Vitro Propagation of Two Tuberous Medicinal Plants: Holostemma ada-kodien and Ipomoea mauritiana	p. 81
In Vitro Production of Gymnemic Acid from Gymnema sylvestre (Retz) R. Br. Ex Roemer and Schultes Through Callus Culture Under Abiotic Stress Conditions	p. 93
Establishment of Plant Regeneration and Cryopreservation System from Zygotic Embryo-Derived Embryogenic Cell Suspension Cultures of Ranunculus kazusensis	p. 107
In Vitro Culture and Secondary Metabolite Isolation in Bryophytes	p. 117
Micropropagation and In Vitro Conservation of Vanilla (Vanilla planifolia Andrews)	p. 129
Protocol for In Vitro Regeneration and Marker Glycoside Assessment in Swertia chirata Buch-Ham	p. 139
Protocols for Establishment of an In Vitro Collection of Medicinal Plants in the Genus Scutellaria	p. 155
Protocols for In Vitro Culture and Phytochemical Analysis of Phyllanthus Species (Euphorbiaceae)	p. 167
In Vitro Clonal Propagation of Asparagus racemosus, a High Value Medicinal Plant	p. 179
Micropropagation of Penthorum chinense Through Axillary Buds	p. 191
Transgenic Approaches
Spontaneous Plant Regeneration and Production of Secondary Metabolites from Hairy Root Cultures of Centaurium erythraea Rafn	p. 205
Transgenic Hypericum perforatum	p. 217
Agrobacterium-Mediated Transformation of Ruta graveolens L	p. 235
Gene Expression Profiling in Taxus baccata L. Seedlings and Cell Cultures	p. 249
Catapol Production in Chinese Foxglove (Rehmannia glutinosa Libos.) Hairy Roots Transformed with Agrobacterium rhizogenes ATCC15834	p. 263
Molecular Markers and Microsatellites
Identification of Medicinal Plants and Plant Sequences: A Multiplexed MLPA Assay	p. 277
Isolation of Microsatellites from Catharanthus roseus (L.) G. Don Using Enriched Libraries	p. 289
Biotransformation, Bioreactors, and Metabolomics
Production of Cinnamyl Glycosides in Compact Callus Aggregate Cultures of Rhodiola rosea Through Biotransformation of Cinnamyl Alcohol	p. 305
Spearmint Plantlet Culture System as a Means to Study Secondary Metabolism	p. 313
Bioreactor Production of Secondary Metabolites from Cell Cultures of Periwinkle and Sandalwood	p. 325
Camptothecin Production by In Vitro Cultures and Plant Regeneration in Ophiorrhiza Species	p. 337
Metabolomic Analysis of Ocotea odorifera Cell Cultures: A Model Protocol for Acquiring Metabolite Data	p. 347
The Production of 9-methoxycanthin-6-one from Callus Cultures of (Eurycoma longifolia Jack) Tongkat Ali	p. 359
Altered Gravity and Biotechnology
Plant Secondary Metabolism in Altered Gravity	p. 373
The Role of Biotechnology in the Production of the Anticancer Compound Podophyllotoxin	p. 387
Index	p. 403
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