Protocols for in Vitro Propagation of Ornamental Plants

by Jain, S. Mohan; Ochatt, Sergio J.

• ISBN13: 9781603273909
• ISBN10: 1603273905
• Format: Hardcover
• Copyright: 2010-01-13
• Publisher: Humana Pr Inc

Summary

While ornamental plants are produced mainly for their aesthetic value, the propagation and improvement of quality attributes such as leaf types, flower colour and fragrance, longevity and form, plant shape and architecture, and the creation of novel variation are important economic goals for the expanding ornamental industry. In Protocols for In Vitro Propagation of Ornamental Plants, leading researchers in the field compile step-wise protocols for rapid plant multiplication and in vitro storage of major commercially viable ornamental plants. Divided into two sections, Section A contains chapters mainly on micropropagation of cut and pot flowers with detailed protocols involving in vitro culture-explants, medium preparation, detailed medium table, shoot initiation and proliferation, root induction, in vitro plant hardening, and field transfer, and Section B delves into reviews on topics such as in vitro production of sweet peas, the status of transgenics in ornamental plants, in vitro conservation, the status of floriculture in Europe, azalea phylogeny, and thin cell layers. As part of the highly successful Methods in Molecular Biology'„¢ series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and clear, Protocols for In Vitro Propagation of Ornamental Plants presents key techniques that will be of great use to floriculturists, researchers, commercial companies, biotechnologists, and students dealing with ornamental plants.

Table of Contents

Foreword	p. v
Preface	p. vii
Contributors	p. xi
Protocols for In Vitro Propagation of Ornamental Plants
Micropropagation and Organogenesis of Anthurium andreanum Lind cv Rubrun	p. 3
A Highly Efficient Protocol for Micropropagation of Begonia tuberous	p. 15
Micropropagation of Vanda teres (Roxb.) Lindle	p. 21
In vitro Propagation Through Axillary Shoot Culture of Ranunculus asiaticus L	p. 29
Micropropagation of Crataeva adansonii D.C. Prodr: An Ornamental Avenue Tree	p. 39
Strategies for the Micropropagation of Bromeliads	p. 47
Micropropagation of Poinsettia by Organogenesis	p. 67
Micropropagation of Phalaenopsis Blume	p. 77
Genetic Transformation of Carnation (Dianthus caryophylus L.)	p. 87
Matrix Supported Liquid Culture and Machine Vision Analysis of Regenerated Shoots of Gladiolus	p. 97
In Vitro Propagation of Carnation (Dianthus caryophyllus L.)	p. 109
In Vitro Propagation of Jasminum officinale L.: A Woody Ornamental Vine Yielding Aromatic Oil from Flowers	p. 117
Micropropagation of Lysionotus pauciflorus Maxim. (Gesneriaceae)	p. 127
Micropropagation of Rhododendron	p. 141
In Vitro Regeneration and Multiplication of Passiflora Hybrid "Guglielmo Betto"	p. 153
In Vitro Propagation of Rose	p. 163
In Vitro Propagation of Chrysanthemum	p. 177
Micropropagation of Codiaeum variegatum (L.) Blume and Regeneration Induction via Adventitious Buds and Somatic Embryogenesis	p. 187
Methods for In Vitro Propagation of Pelargonium x hortorum and Others: From Meristems to Protoplasts	p. 197
Marigold Regeneration and Molecular Analysis of Carotenogenic Genes	p. 213
Somatic Embryogenesis in In Vitro Culture of Leucojum vernum L.	p. 223
In Vitro Propagation of Australian Native Ornamental Plant, Scaevola	p. 235
Micropropagation of Tulip: Production of Virus-Free Stock Plants	p. 243
In Vitro Propagation of Ornamental Myrtus (Myrtus communis)	p. 257
Sunflower Propagation	p. 271
Clonal Propagation of Cyclamen persicum Via Somatic Embryogenesis	p. 281
Protocols for In Vitro Propagation of Ornamental Plants
In Vitro Production of Sweet Peas (Lathyrus odoratus L.) via Axillary Shoots	p. 293
In Vitro Conservation and Cryopreservation of Ornamental Plants	p. 303
Genetic Engineering of Novel Flower Colors in Floricultural Plants: Recent Advances via Transgenic Approaches	p. 325
Azalea Phylogeny Reconstructed by Means of Molecular Techniques	p. 349
Status of Floriculture in Europe	p. 365
Thin Cell Layers: Power-Tool for Organogenesis of Floricultural Crops	p. 377
Index	p. 393
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