Recombinant DNA

by Kaplan; Colowick; Wu

• ISBN13: 9780121819682
• ISBN10: 012181968X
• Format: Hardcover
• Copyright: 1980-03-28
• Publisher: Elsevier Science

Summary

The critically acclaimed laboratory standard, Methods in Enzymologylt;/b>, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Table of Contents

Vectors for Cloning DNA: Production of Single-Stranded Plasmid DNA

pKUN, Vectors for the Separate Production of Both DNA Strands of Recombinant Plasmids

Restriction Site Bank Vectors for Cloning in Gram-Negative Bacteria and Yeast

Plasmids for the Selection and Analysis of Prokaryotic Promoters

A *gl DNA Protocol Based on Purification of Phage on DEAE-Cellulose

Double cos Site Vectors: Simplified Cosmid Cloning

Improved in Vitro Packaging of *gl DNA

*gl Phage Vectors--Embl Series

Plasmid and Phage Vectors for Gene Cloning and Analysis in Streptomyces

Cosmid Shuttle Vectors for Cloning and Analysis of Streptomyces DNA

Host-Vector Systems for Gene Cloning in Cyanobacteria

Genetic Engineering of the Cyanobacterial Chromosome

Conjugal Transfer of Plasmids to Cyanobacteria

Site-Directed Chromosomal Rearrangements in Yeast

Improved Vectors for Plant Transformation

Expression Cassette Vectors and New Selectable Markers

Vectors for Cloning in Plant Cells

Binary Ti Vectors for Plant Transformation and Promoter Analysis

Detection for Monocot Transformation via Agrobacterium tumefaciens

Direct Gene Transfer to Protoplasts of Dicotyledonous and Monocotyledonous Plants by a Number of Methods, Including Electroporation

Uptake of DNA and RNA into Cells Mediated by Electroporation

Electroporation of DNA and RNA into Plant Protoplasts

Cloning Vectors of Mitochondrial Origin for Eukaryotes

Vectors for Expression of Cloned Genes

Short Homopeptide Leader Sequences Enhanced Production of Human Proinsulin in Escherichia coli

Expression of Bovine Growth Hormone Derivatives in Escherichia coli and the Use of the Derivatives to Produce Natural Sequence Growth Hormone

Expression of Eukaryotic Genes in Escherichia coli with a Synthetic Two-Cistron System

Expression of Heterologous Unfused Protein in Eshcerichia coli

Directing Ribosomes to a Single mRNA Species

A Method to Study Ribosomal RNA Mutations and Their Effects on Translation of a Single Messenger in Escherichia coli

New Expression Vectors for Identifying and Testing Signal Structures for Initiation and Termination of Transcription

Synthesis and Sequence-Specific Proteolysis of Hybrid Proteins Produced in Escherichia coli

Expression Plasmid Containing the *gl PL Promoter and cI857 Repressor

Expression and Secretion of Foreign Proteins in Escherichia coli

Engineering for Protein Secretion in Gram-Positive Bacteria

Expression and Secretion Vectors for Yeast

Vaccinia Virus as an Expression Vector

Author Index

Subject Index

Table of Contents provided by Publisher. All Rights Reserved.