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9780127655604

Recombinant DNA Methodology

by ; ;
  • ISBN13:

    9780127655604

  • ISBN10:

    0127655603

  • Edition: 1st
  • Format: Paperback
  • Copyright: 1989-06-28
  • Publisher: Elsevier Science

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Summary

Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. Key Features * Enzymes in DNA research * DNA isolation, hybridization, and cloning * DNA sequence analysis * cDNA cloning * Gene products * Identification of cloned genes and mapping of genes * Monitoring cloned gene expression * Cloning and transferring of genes into yeast cells * Cloning and transferring of genes into plant cells * Cloning and transferring of genes into animal cells * Site-directed mutagenesis * Protein engineering * Expression vectors

Table of Contents

Enzymes in Recombinant DNA Research
DNA-Joining Enzymes: A Review
Guide to the Use of Type II Restriction Endonucleases
Site-Specific Cleavage of DNA at 8-, 9- and 10bp Sequences
Exonuclease III: Use for DNA Sequence Analysis and in Specific Deletions of Nucleotides
Methods for Isolation, Purification, or Amplification of DNA
Gel Electrophoresis of Restriction Fragments
Purification, Specific Fragmentation, and Separation of Large DNA Molecules
Orthogonal-Field-Alternation Gel Electrophoresis
A Rapid Alkaline Extraction Method for the Isolation of Plasmid DNA
Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction
Vectors or Methods for Gene Cloning
Escherichia coli Plasmids Packageable in Vitro in a
Table of Contents provided by Publisher. All Rights Reserved.

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