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9780122497001

Rna Methodologies

by
  • ISBN13:

    9780122497001

  • ISBN10:

    0122497007

  • Format: Paperback
  • Copyright: 1993-01-01
  • Publisher: Academic Pr
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Supplemental Materials

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Summary

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA. * Studies cellular regulation using RNA as a parameter of Gene Expression * Offers RNA isolation strategies * Explains proper handling, storage, and manipulation of RNA

Table of Contents

RNA and the Cellular Biochemistry
Revisited Transcription and the Organization of Eukaryotic Genes
Messenger RNA Resilient
Ribonucleases RNA Isolation Strategies
The Truth About Tissues
Isolation of Polyadenylated RNA
Quality Control for RNA Preparations
Dot Blot Analysis
Electrophoresis of RNA
Photodocumentation and Image Analysis
Northern Analysis
Nucleic Acid
Probe Technology
Practical Nucleic Acid
Hybridization
Principles of Detection
Quantification of Specific mRNAs by Nuclease
Protection Analysis of Nuclear RNA
cDNA Synthesis
RT-PCR Quantitative
PCR Techniques
Transcript Subtraction Methods
mRNA Differential Display
High Throughput Analysis of Gene Expression
RNA Interference Genomes, Transcriptomes, Proteomes, and Bioinformatics An RNA Paradigm
Table of Contents provided by Publisher. All Rights Reserved.

Supplemental Materials

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