Metalloproteins

This volume describes the roles that metals play throughout biology. Metals are involved in most aspects of the chemistry of life, including respiration, numerous steps of metabolism, photosynthesis, nitrogen fixation, nerve transmission, signal transduction, muscle contraction, oxygen transport, and protection from xenobiotic compounds. In addition, metals are used in medicine as therapeutic agents. In twelve chapters, several of the best-known experts in their specialties explain the participation of metals in a variety of biological functions while teaching principles of chemistry and offering perspectives on the future of research in bioinorganic chemistry.Although an ideal text for upper-level undergraduates, graduate students, and their teachers, this book also offers enlightening reading to chemists, biochemists, and biological scientists who specialize in other fields but want to know what is happening in contemporary bioinorganic chemistry research. The topics covered include how bacteria rid themselves of potentially toxic metals, the biological and chemical aspects of metalloproteins involved in oxygen transport, the use of metal-containing drugs in treating various types of cancer, and the basics of biological electron transfer reactions.The contributors are Barry P. Rosen, Susan M. Miller, Eric D. Coulter, Issa S. Isaac, John H. Dawson, Stephen G. Sligar, Donald M. Kurtz, Jr., A. Grant Mauk, Russ Hille, E. Neil,G. Marsh, James W. Whittaker, Joan B. Broderick, Elizabeth E. Trimmer, and John M. Essigmann.

Preface

xi

Authors

xv

Abbreviations

xix

The role of efflux in bacterial resistance to soft metals and metalloids

Barry P. Rosen

Introduction

1

(2)

Soft-metal P-type ATPases

3

(2)

Resistance to Cu(I) and Ag(I) --- CopA and CopB

5

(2)

Resistance to Zn(II) and Cd(II) --- CadA and ZntA

7

(1)

Resistance to Zn(II) and Cd(II) --- CzcCBA

8

(1)

Resistance to arsenic and antimony

9

(3)

Conclusions

12

(1)

Summary

13

(1)

References

13

(4)

Bacterial detoxification of Hg(II) and organomercurials

Susan M. Miller

Introduction

17

(1)

Overview of mer operons and roles of proteins

17

(1)

Regulation of gene expression --- merR

18

(4)

Proteins of mercury transport

22

(2)

Organomercurial resistance

24

(1)

The key step --- Hg(II) reduction

25

(3)

Perspectives

28

(1)

Summary

28

(1)

References

29

(2)

Non-heam iron-containing oxygenases involved in the microbial biodegradation of aromatic hydrocarbons

Eric D. Coulter

David P. Ballou

Introduction

31

(2)

Oxygen activation at non-haem iron centres

33

(1)

Catechol dioxygenases

34

(2)

Mononuclear Fe(II) dioxygenases (Rieske oxygenases)

36

(5)

Fe(II)-pterin-dependent hydroxylases

41

(2)

Di-iron-oxo mono-oxygenases

43

(3)

Conclusions

46

(1)

Perspectives

46

(1)

Summary

47

(1)

References

47

(4)

Haem iron-containing peroxidases

Issa S. Isaac

John H. Dawson

Introduction

51

(3)

Compound I

54

(8)

Compound II

62

(1)

Compound III

63

(1)

Compound O

63

(1)

Differences in the catalytic activities of P450 and peroxidases

64

(1)

Perspectives

65

(1)

Summary

66

(1)

References

67

(4)

Nature's universal oxygenases: the cytochromes P450

Stephen G. Sligar

Introduction

71

(1)

Classification of P450s

72

(4)

Mechanism

76

(6)

Perspectives

82

(1)

Summary

82

(1)

References

82

(3)

Oxygen-carrying proteins: three solutions to a common problem

Donald M. Kurtz, Jr.

Introduction

85

(1)

Reactivity of molecular oxygen with transition-metal ions

86

(1)

The active sites of oxygen-carrying proteins

87

(3)

Hb and Mb

90

(3)

Hr and myoHr

93

(2)

Hcy

95

(2)

Perspectives

97

(1)

Summary

98

(1)

References

99

(2)

Biological electron-transfer reactions

A. Grant Mauk

Introduction

101

(1)

Inorganic origins

102

(1)

Theoretical basics

103

(3)

Electrochemistry and biological electron-transfer kinetics

106

(7)

Early biological kinetic experiments

113

(3)

Refinements of metalloprotein electron-transfer experiments

116

(2)

New challenges concerning intramolecular electron transfer

118

(1)

Protein-protein electron-transfer reactions

119

(2)

An outlook

121

(1)

Summary

121

(1)

References

122

(3)

Molybdenum enzymes

Russ Hille

Introduction

125

(1)

Classification of molybdenum enzymes

126

(1)

The molybdenum hydroxylases

127

(4)

The eukaryotic molybdenum oxotransferases

131

(2)

The bacterial molybdenum oxotransferases and related enzymes

133

(1)

Perspectives

134

(2)

Summary

136

(1)

References

136

(3)

Coenzyme B12 (cobalamin)-dependent enzymes

E. Neil G. Marsh

Introduction

139

(1)

Structures of B12 coenzymes

140

(1)

Interaction of B12 with proteins

141

(2)

AdoCbl-dependent isomerases

143

(4)

AdoCbl-dependent riboucleotide reductase

147

(1)

MeCbl-dependent enzymes

148

(3)

Perspectives

151

(1)

Summary

152

(1)

References

153

(2)

Oxygen reactions of the copper oxidases

James W. Whittaker

Introduction

155

(1)

O2 redox chemistry

156

(3)

Biological chemistry of O2

159

(1)

Copper oxidases

160

(1)

Role of copper

161

(1)

Role of the redox cofactor

162

(2)

Dioxygen reaction with the active site

164

(4)

Oxygen reactions in cofactor biogenesis

168

(2)

Summary

170

(1)

References

170

(3)

Catechol dioxygenases

Joan B. Broderick

Introduction

173

(3)

Sources of the enzymes and role in biormediation

176

(1)

Intradiol catechol dioxygenases

177

(6)

Extradiol catechol dioxygenases

183

(3)

Perspectives

186

(1)

Summary

186

(1)

References

187

(4)

Cisplatin

Elizabeth E. Trimmer

John M. Essigmann

Introduction

191

(2)

DNA adducts formed by cis- and trans-DDP

193

(4)

Effects of DNA replication and transcription

197

(1)

Repair of platinum adducts

198

(1)

Recognition of platinum adducts by cellular proteins

199

(5)

Mechanisms of cisplatin resistance

204

(4)

Conclusions and outlook

208

(1)

Summary

209

(1)

Rent More, Save More! Use code: ECRENTAL

Rent More, Save More! Use code: ECRENTAL

5% off 1 book, 7% off 2 books, 10% off 3+ books

9780691050485

0691050481

Summary

Table of Contents

Supplemental Materials

Rewards Program

Rent More, Save More! Use code: ECRENTAL

Rent More, Save More! Use code: ECRENTAL

5% off 1 book, 7% off 2 books, 10% off 3+ books

Metalloproteins

9780691050485

0691050481

Summary

Table of Contents

Supplemental Materials

Rewards Program

Digital License