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9783540705703

Standardization and Quality Assurance in Fluorescence Measurements II

by ; ; ; ;
  • ISBN13:

    9783540705703

  • ISBN10:

    3540705708

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2008-08-01
  • Publisher: Springer Verlag
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Summary

The validation and standardization of fluorescence methods is still in its infancy as compared to other prominent analytical and bioanalytical methods. Appropriate quality assurance standards are however a prerequisite for applications in highly regulated fields such as medical diagnostics, drug development, or food analysis.

Table of Contents

Fluorescence Microscopy
Need for Standardization of Fluorescence Measurements from the Instrument Manufacturer's Viewp. 3
Characterization and Calibration in Wide Field and Sectioned Fluorescence Microscopy SIPchartsp. 25
Quantitative Fluorescence Microscopy: Considerations and Controlsp. 55
Comparability of Fluorescence Microscopy Data and Need for Instrument Characterization of Spectral Scanning Microscopesp. 89
Fluorescence Lifetime Imaging Microscopy: Quality Assessment and Standardsp. 117
Single Molecule Spectroscopy
State of the Art and Novel Trends in Fluorescence Correlation Spectroscopyp. 145
Single Molecule Spectroscopy: Instrumentation and Multiparameter Detectionp. 199
Fluorescence-Based Microarray Technology: Applications, Future Trends, and Need for Standardization
DNA Microarrays: Applications, Future Trends, and the Need for Standardizationp. 215
Comparability of Microarray Experiments from the Instrument and the Sample Site and Approaches Towards Standardizationp. 239
Microarray Technology: Unresolved Issues and Future Challenges from a Regulatory Perspectivep. 265
Protein Arrays and Fluorescence Detection: Applications and Limitationsp. 283
Flow Cytometry
Flow Cytometry: Instrumentation, Applications, Future Trends and Limitationsp. 307
Flow Cytometry Quality Assurancep. 343
Approaches to Quantitation in Flow Cytometryp. 371
Fluorescence Immunoassays
Immunoassays: Basic Concepts, Physical Chemistry and Validationp. 401
Time-Resolved Fluorometric Immunoassays; Instrumentation, Applications, Unresolved Issues and Future Trendsp. 429
Particle-Based Assays: Applications and Unresolved Issuesp. 449
Advances in Fluorescence Enzyme Detection Methodsp. 469
Quantitative PCR
Quantitative Real-Time PCR: Fluorescent Probe Options and Issuesp. 489
Fluorescence In Situ Hybridization and Immunohistochemistry
Cellular Bioimaging in Fluorescent Cancer Biomarker Evaluation: Validation, Technologies and Standards Developmentp. 511
Fluorescence Technologies in Biomedical Diagnostics
Fluorescence Techniques in Biomedical Diagnostics: Instrumentation, Analysis and Unresolved Issuesp. 533
In-vivo Fluorescence Imaging: Applications, Future Trends & Approaches to Standardizationp. 549
Subject Indexp. 561
Table of Contents provided by Ingram. All Rights Reserved.

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