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9780306482304

Peptide Nucleic Acids, Morpholinos, And Related Antisense Biomolecules

by ;
  • ISBN13:

    9780306482304

  • ISBN10:

    0306482304

  • Format: Hardcover
  • Copyright: 2006-03-20
  • Publisher: Landes Bioscience

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

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Supplemental Materials

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Summary

This volume is unique to the existing literature in the Peptide Nucleic Acid field, in that it focuses on comparing and contrasting PNA with other available oligonucleotide homologues and considers areas in which these biomolecules could be profitably applied to clinical and diagnostic applications. Part I of the book addresses comparative strengths and weaknesses of various nucleoside homologues. Part II of the book addresses specific translational or clinical applications for PNA and related antisense biomolecules. The editors have succeeded in presenting a balanced yet broad view of the methods available for gene targeting and modification.

Table of Contents

Preface xvii
Part I Research Applications
1. The Many Faces of PNA
3(15)
Peter E. Nielsen
Introduction to PNA
3(1)
PNA Chemistry
3(1)
Cellular Uptake of PNA
4(1)
Antisense Applications
5(1)
Antigene Properties
5(4)
Antimicrobial PNAs
9(2)
Genetic Information Carrier
11(1)
PNA in Diagnostics
12(1)
Prospects
13(5)
2. Modulation of Nucleic Acid Information Processing by PNAs: Potential Use in Anti-Viral Therapeutics
18(12)
Lionel Bastide, Bernard Lebleu and Ian Robbins
PNAs as Gene-Modulator Agents
21(4)
Virus Specific Nucleic Acid Processing
25(1)
PNA-Tuning
26(4)
3. Targeted Gene Delivery: The Role of Peptide Nucleic Acid
30(8)
Kenneth W Liang, Feng Liu and Leaf Huang
Targeted Gene Delivery through Polycation/DNA Complex
31(2)
Targeted Delivery of Naked DNA
33(5)
4. Imaging Gene Expression in the Brain with Peptide Nucleic Acid (PNA) Antisense Radiopharmaceuticals and Drug Targeting Technology
38(23)
Ruben J. Boado and William M. Pardridge
Mechanism of Action of Antisense Drugs
40(1)
Medical Diagnostic and Therapeutic Applications
41(4)
Functional Genomics
45(1)
Overview of Antisense Molecules and Effective Delivery
45(2)
Brain Drug Targeting Systems
47(5)
Physiological Brain Efflux of IgG
52(1)
Imaging of Brain Gene Expression
52(9)
5. Receptor-Specific Targeting with Complementary Peptide Nucleic Acids Conjugated to Peptide Analogs and Radionuclides
61(28)
Eric Wickstrom, Mathew L. Thakur and Edward R. Sauter
Synthesis of Probes
68(3)
Applications in Cells
71(5)
Applications in Animals
76(4)
Applications in Patients
80(9)
6. Morpholinos and PNAs Compared
89(25)
James E. Summerton
Classification of Antisense Structural Types
90(1)
Preparation of Morpholinos and PNAs
91(2)
Properties of Morpholinos and PNAs
93(13)
Applications
106(8)
7. Chemistry of Locked Nucleic Acids (LNA): Design, Synthesis and Bio-Physical Properties
114(19)
Jesper Wengel, Michael Petersen, Miriam Frieden and Troels Koch
Synthesis of LNA Monomers
114(1)
Solid Phase Synthesis of LNA Oligonucleotides
115(3)
Hybridization Characteristics of LNA Oligonucleotides
118(1)
Hybridization Kinetics of LNA Oligonucleotides
119(3)
Structure of LNA Oligonucleotides
122(1)
DNA and RNA Structure
122(1)
LNA Structure
123(1)
LNA:RNA Duplexes
123(2)
LNA:DNA Duplexes
125(1)
LNA:LNA Duplexes
126(1)
α-L-LNA:RNA Duplexes
126(1)
α-L-LNA:DNA Duplexes
126(1)
Implications for RNase H Activity of LNA:RNA and α-L-LNA:RNA Duplexes
127(1)
Stability of LNA and α-L-LNA Modified Nucleic Acids
127(1)
LNA Triplexes
128(5)
8. Recent Applications of RNA Interference (RNAi) in Mammalian Systems
133(18)
Lisa Scherer and John J. Rossi
siRNA Design
133(4)
Recent Applications of RNAi
137(5)
Functional Genomics
142(1)
RNAi versus Ribozymes
143(8)
Part II Clinical Applications
9. Peptide Nucleic Acids as Epigenetic Inhibitors of HIV-1
151(20)
Shizuko Sei
HIV-1 Life Cycle and Potential Molecular Targets
152(1)
Genetic Strategies to Inhibit HIV-1 Replication
153(1)
Peptide Nucleic Acids as Epigenetic HIV-1 Inhibitors
154(7)
Potential Use of PNA Against Other Infectious Pathogens
161(1)
Bio-Delivery of PNA
161(2)
Future Perspectives
163(8)
10. Therapeutic Uses of Peptide Nucleic Acids (PNA) in Oncology
171(10)
Nadia Zaffaroni, Raffaella Villa and Marco Folini
Potential of PNAs as Tools for Anticancer Therapeutic Interventions
172(6)
Perspectives
178(3)
11. PNAs as Novel Cancer Therapeutics
181(14)
Luca Mologni and Carlo Gambacorti-Passerini
Biochemistry of Peptide Nucleic Acids
182(1)
PNA as a Biomolecular Tool
183(1)
Antisense and Anti-Gene Properties of PNA
184(5)
Future Directions
189(6)
12. Medicinal Chemistry of Plasmid DNA with Peptide Nucleic Acids: A New Strategy for Gene Therapy
195(17)
Olivier Zelphati, Jiin Feigner, Yan Wang, Xiaowu Liang, Xiaodong Wang and Philip Felgner
Principle of PNA Dependent Gene Chemistry Technology
197(1)
Labeling of Plasmid DNA to Study Gene Delivery Mechanism
198(1)
Transition
199(1)
Overcoming the Barriers to Improve Gene Delivery and Expression
200(1)
PNA-Conjugates for Targeting DNA to Cell Surface Receptors
201(1)
PNA-Peptide Conjugates to Overcome Cell Membrane Barriers
202(4)
Other Potential Applications of PNA Conjugate for Gene Delivery
206(6)
13. Locked Nucleic Acids (LNA) and Medical Applications
212(11)
Henrik Ørum, Andreas Wolter and Lars Kongsbak
Biochemistry of LNA
212(3)
LNA in Diagnostics and Genomics Applications
215(3)
LNA in Therapeutic Applications
218(3)
Future Perspectives on LNA
221(2)
14. Peptide Nucleic Acids as Agents to Modify Target Gene Expression and Function
223(13)
Gan Wang and Peter M. Glazer
PNA Binding Affinity
224(1)
PNA Binding Specificity
224(1)
Detection of PNA Binding-Induced Transcription in Hela Nuclear Extract in Vitro Transcription System
224(2)
Determination of the Initiation Sites of PNA Binding-Induced Transcription
226(2)
PNA Binding-Generated D-Loops Lead to GFP Gene Expression in Mammalian Cells
228(1)
PNA-Induced Endogenous y-Globin Gene Expression in Human Cells
228(3)
The Correlation between PNA Binding-Generated D-Loops and the Natural Promoter of the Gene in Target Gene Transcription 229 \
The PNA Length Requirement for Inducing Transcription from the PNA Binding Sites
231(1)
Transcription Components Involved in PNA Binding-Induced Transcription
232(1)
The Limitation of PNA Binding-Induced Target Gene Expression
233(1)
PNAs for Targeted Genome Modification
234(2)
15. Peptide Nucleic Acids: Cellular Delivery and Recognition of DNA and RNA Targets
236(7)
David R. Corey
Hybridization by PNA: Affinity Isn't Everything
236(2)
Strand Invasion by PNAs
238(1)
Intracellular Delivery of PNAs
239(1)
Applications for PNAs Delivered within Cells
240(3)
16. The Use of PNAs and Their Derivatives in Mitochondrial Gene Therapy
243(9)
Paul M. Smith, Günther F. Ross, Theresa M. Wardell, Robert W. Taylor, Douglass M. Turnbull and Robert N. Lightowlers
The Antigenomic Hypothesis
244(1)
PNA as the Choice of Antigenomic Agent
244(2)
Cellular Uptake and the Problem of Mitochondrial Import
246(1)
PNAs as Antigenomic Molecules – Do They Work?
247(1)
Trouble-Shooting the Antigenomic Approach to mtDNA Disease
248(4)
17. Gene Silencing through RNA Interference: Potential for Therapeutics and Functional Genomics
252(13)
David O. Azorsa, Spyro Mousses and Natasha J. Caplen
RNAi: An Historical Perspective
252(1)
RNAi: Summary of Mechanism
253(1)
Physiological Role of RNAi and Related Post-Transcriptional Gene Silencing Mechanisms
253(2)
RNAi in Mammalian Cells
255(1)
The Delivery of Triggers of RNAi
256(1)
RNAi as a Functional Genomics Tool
257(1)
RNAi as a Therapeutic Strategy
258(7)
18. Transcriptional Activation of Human CREB Gene Promoter Using Bis-PNA (Peptide Nucleic Acid)
265(6)
Christopher G. Janson, Matthew J. During, Yelena Shifman and Paola Leone
Materials and Methods
266(1)
Results
267(1)
Future Directions for Trans-Activation in the Brain
268(3)
Index 271

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