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9781588296795

Tissue Proteomics

by ;
  • ISBN13:

    9781588296795

  • ISBN10:

    1588296792

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2008-03-01
  • Publisher: Humana Pr Inc

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Summary

In one volume this book provides useful and innovative protocols developed specifically for the proteomic profiling of human tissues. The book provides high-throughput gel-based techniques, microarrays and a number of other methods used in proteomic research. This important book will prove indispensable to investigators of biomarker discovery and therapeutic response profiling, as well as those forging new paths in the fields of theranostics and personalized medicine.

Table of Contents

Prefacep. v
Contributorsp. ix
Capabilities Using 2-D DIGE in Proteomics Research: The New Gold Standard for 2-D Gel Electrophoresisp. 1
Protein Carbohydrate Analysis: Gel-Based Staining, Liquid Chromatography, Mass Spectrometry, and Microarray Screeningp. 19
Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry for Protein and Peptide Profiling of Body Fluidsp. 41
A Novel Approach Using MALDI-TOF/TOF Mass Spectrometry and Prestructured Sample Supports (AnchorChip Technology) for Proteomic Profiling and Protein Identificationp. 57
Automated Laser Capture Microdissection for Tissue Proteomicsp. 71
Tissue Microarrays: An Overviewp. 91
Frozen Protein Arraysp. 105
Reverse Phase Protein Microarrays for Theranostics and Patient-Tailored Therapyp. 113
Antibody Arrays for Determination of Relative Protein Abundancesp. 129
SH2 Domain-Based Tyrosine Phosphorylation Arrayp. 153
Immunoregulomics: A Serum Autoantibody-Based Platform for Transcription Factor Profilingp. 163
The "Reverse Capture" Autoantibody Microarray: An Innovative Approach to Profiling the Autoantibody Response to Tissue-Derived Native Antigensp. 175
Biorepository Standards and Protocols for Collecting, Processing, and Storing Human Tissuesp. 193
Indexp. 221
Table of Contents provided by Ingram. All Rights Reserved.

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