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9780896036987

Angiogenesis Protocols

by
  • ISBN13:

    9780896036987

  • ISBN10:

    0896036987

  • Format: Hardcover
  • Copyright: 2001-03-01
  • Publisher: Springer Verlag

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Summary

A comprehensive collection of key techniques for assessing the formation of new blood vessels in both whole tissues and cell culture. These readily reproducible methods range from low-cost in vitro assays to cutting-edge in vivo models, such as the dorsal window chamber, which demands high levels of surgical skill as well as relatively expensive, custom-made equipment. Timely and highly practical, Angiogenesis Protocols is a gold-standard resource for today's angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources.

Table of Contents

Preface v
Contributors ix
I Special Review Article
Therapeutic Inhibition of Angiogenesis
3(26)
Hua-Tang Zhang
Roy Bicknell
II Angiogenesis Protocols In Vivo
Microscopic Assessment of Angiogenesis in Tumors
29(18)
Stephen B. Fox
In Vivo Matrigel Migration and Angiogenesis Assays
47(6)
Katherine M. Malinda
Alginate Microbead Release Assay of Angiogenesis
53(6)
Clifford Y. Ko
Vivek Dixit
William W. Shaw
Gary Gitnick
Disc Angiogenesis Assay
59(18)
Anthony C. Allison
Luis-F. Fajardo
Sponge Implant Model of Angiogenesis
77(10)
Silvia P. Andrade
Hollow Fiber Assay for Tumor Angiogenesis
87(8)
Roger M. Phillips
Michael C. Bibby
Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
95(12)
Axel Sckell
Michael Leunig
Angiogenesis Assays Using Chick Chorioallantoic Membrane
107(24)
David C. West
W. Douglas Thompson
Paula G. Sells
Mike F. Burbridge
Corneal Assay for Angiogenesis
131(14)
Marina Ziche
III Angiogenesis Protocols In Vitro
Collagen Gel Assay for Angiogenesis: Induction of Endothelial Cell Sprouting
145(18)
Ana M. Schor
Ian Ellis
Seth L. Schor
Chemotaxis and Chemokinesis in 3D Macromolecular Matrices: Relevance to Angiogenesis
163(22)
Ana M. Schor
Ian Ellis
Seth L. Schor
Rat Aortic Ring: 3D Model of Angiogenesis In Vitro
185(20)
Mike F. Burbridge
David C. West
In Vitro Matrigel Angiogenesis Assays
205(8)
M. Lourdes Ponce
IV Associated Techniques
Microvessel Endothelial Cells from Human Adipose Tissues: Isolation, Identification, and Culture
213(14)
Peter W. Hewett
Transfection and Transduction of Primary Human Endothelial Cells
227(10)
Stewart G. Martin
Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
237(10)
Richard C. M. Siow
Jeremy D. Pearson
Bovine Retinal Microvascular Pericytes: Isolation, Propagation, and Identification
247(18)
Ramesh C. Nayak
Ira M. Herman
Index 265

Supplemental Materials

What is included with this book?

The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

The Used, Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.

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