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9780387321219

Color Atlas of Immunocytochemistry in Diagnostic Cytology

by ;
  • ISBN13:

    9780387321219

  • ISBN10:

    0387321217

  • Edition: 1st
  • Format: Paperback
  • Copyright: 2006-10-13
  • Publisher: Springer Nature

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Summary

In recent years, cytology has played an increasingly important role in the diagnosis of disease, particularly those of neoplastic origin. It is not unusual for cytologic specimens to be the only diagnostic samples available from patients with cancer. Many ancillary tests traditionally performed on histologic material are now being performed on cytologic specimens. One such technique, immunocytochemistry (ICC), has already proved to be important in diagnostic tumor pathology.This atlas examines the value of ICC as well as its limitations in contemporary cytology. Methods have been refined and high-quality reagents are now available. As a result, technical problems no longer present a major concern in this field. This volume will briefly will address the technical aspects of ICC by providing practical advice for the users and analytical aspects of ICC, including correct selection of markers for specific differential diagnoses and incorporation of results in final cytologic interpretation; illustrate the utilization of ICC in the resolution of some common diagnostic problems in fine needle aspiration and body cavity fluid cytology. ICC of undifferentiated malignant neoplasms, carcinomas of unknown origin, and most importantly its utilization in specific differential diagnoses that are based on cytomorpholoy and patient's clinical history is also discussed. Finally, the value and limitations of ICC in the detection of predictive markers of cancer and potential treatment targets will be reviewed. This practical handbook will allow for quick reference in the selection and interpretation of markers in specific differential diagnostic in the daily practice of diagnostic cytology.

Author Biography

Parvin Ganjei-Azar, MD is Professor of Pathology and Director of Cytopathology at the University of Miami, Jackson Memorial Hospital in Miami, Florida.Mehrdad Nadji, MD is Professor of Pathology and Director of Immunohistochemistry at the University of Miami, Jackson Memorial Hospital in Miami, Florida.

Table of Contents

Preface vii
Acknowledgments ix
Section I Immunocytochemistry
1 Technical Considerations
3(3)
Specimen
3(1)
Fixation
4(1)
Immunocytochemical Procedure
4(1)
Controls
5(1)
2 Selection of the Markers
6(1)
3 Evaluation of Results
7(4)
False-Positive Reactions
7(1)
False-Negative Reactions
7(1)
Background Staining
7
Section II Immunocytochemical Resolution of Diagnostic Problems: Case Examples
4 Undifferentiated Neoplasms
11(16)
5 Soft Tissue Tumors
27(26)
6 Gastrointestinal Tract
53(14)
7 Pancreas and Liver
67(34)
8 Adrenal Gland
101(16)
9 Head and Neck
117(26)
10 Lung
143(26)
11 Pleura and Mediastinum
169(26)
12 Abdominal Cavity
195(8)
13 Female Genital Tract and Breast
203(30)
14 Urinary and Male Genital System
233(26)
15 Lymphoreticular System
259(14)
16 Nervous System
273(10)
Suggested Readings 283(6)
Index 289

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