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9780896037113

Antibody Phage Display

by ;
  • ISBN13:

    9780896037113

  • ISBN10:

    0896037118

  • Edition: 1st
  • Format: Paperback
  • Copyright: 2001-12-01
  • Publisher: Humana Pr Inc

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Summary

This comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.

Table of Contents

Preface v
Contributors xi
Overview of Antibody Phage-Display Technology and Its Applications
1(38)
Hennie R. Hoogenboom
Standard Protocols for the Construction of Fab Libraries
39(20)
Michelle A. Clark
Standard Protocols for the Construction of scFv Libraries
59(14)
Simon Lennard
Broadening the Impact of Antibody Phage Display Technology: Amplification of Immunoglobulin Sequences from Species Other than Humans or Mice
73(14)
Philippa M. O'Brien
Robert Aitken
Construction of Large Naive Fab Libraries
87(14)
Hans J. W. de Haard
Construction of Polyclonal Antibody Libraries Using Phage Display
101(12)
Jacqueline Sharon
Seshi R. Sompuram
Chiou-Ying Yang
Brent R. Williams
Stefanie Sarantopoulos
Antigen-Driven Stimulation of B-Lymphocytes In Vitro
113(8)
Zhiwei Hu
The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion
121(12)
Ruud M. T. de Wildt
Rene M. A. Hoet
Panning of Antibody Phage-Display Libraries: Standard Protocols
133(14)
David W. J. Coomber
Selection of Antibodies Against Biotinylated Antigens
147(12)
Patrick Chames
Hennie R. Hoogenboom
Paula Henderikx
Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries
159(14)
Keith A. Charlton
Andrew J. Porter
Blocking Immunodominant Epitopes by Competitive Deselection
173(6)
Roberto Burioni
Rescue of a Broader Range of Antibody Specificities Using an Epitope-Masking Strategy
179(8)
Henrik J. Ditzel
Screening of Phage-Expressed Antibody Libraries by Capture Lift
187(8)
Jeffry D. Watkins
Antibody-Guided Selection Using Capture-Sandwich ELISA
195(6)
Kunihiko Itoh
Toshio Suzuki
Proximity-Guided (ProxiMol) Antibody Selection
201(6)
Jane K. Osbourn
Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
207(12)
Mariangela Figini
Silvana Canevari
Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques
219(8)
Don L. Siegel
Isolation of Human Tumor-Associated Cell Surface Antigen-Binding scFvs
227(8)
Elvyra J. Noronha
Xinhui Wang
Soldano Ferrone
Subtractive Isolation of Single-Chain Antibodies Using Tissue Fragments
235(10)
Katarina Radosevic
Willem van Ewijk
Selection of Antibodies Based on Antibody Kinetic Binding Properties
245(10)
Ann-Christin Malmborg
Nina Nilsson
Mats Ohlin
Selection of Functional Antibodies on the Basis of Valency
255(4)
Manuela Zaccolo
Two-Step Strategy for Alteration of Immunoglobulin Specificity by In Vitro Mutagenesis
259(10)
Yoshitaka Iba
Chie Miyazaki
Yoshikazu Kurosawa
Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement
269(18)
Partha S. Chowdhury
Error-Prone Polymerase Chain Reaction for Modification of scFvs
287(8)
Pierre Martineau
Use of Escherichia coli Mutator Cells to Mature Antibodies
295(8)
Robert A. Irving
Gregory Coia
Anna Raicevic
Peter J. Hudson
Chain Shuffling to Modify Properties of Recombinant Immunoglobulins
303(14)
Johan Lantto
Pernilla Jirholt
Yvelise Barrios
Mats Ohlin
Generation of Bispecific and Tandem Diabodies
317(16)
Sergey M. Kipriyanov
High-Level Periplasmic Expression and Purification of scFvs
333(10)
Sergey M. Kipriyanov
Periplasmic Expression and Purification of Recombinant Fabs
343(6)
Robert L. Raffai
Expression of Antibody Fragments in Pichia pastoris
349(10)
Philipp Holliger
Expression of VHH Antibody Fragments in Saccharomyces cerevisiae
359(8)
J. Marcel van der Vaart
Intrabodies: Targeting scFv Expression to Eukaryotic Intracellular Compartments
367(12)
Pascale A. Cohen
Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells
379(10)
Michelle de Graaf
Ida H. van der Meulen-Muileman
Herbert M. Pinedo
Hidde J. Haisma
Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells
389(8)
Pietro P. Sanna
Index 397

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