Cellular Diagnostics : Basic Principles, Methods, and Clinical Applications of Flow Cytometry

by Sack, Ulrich

ISBN13:
9783805585552
ISBN10:
3805585551
Edition: 1st
Format: Hardcover
Copyright: 2008-11-01
Publisher: KARGER, S. PUBLISHERS INC
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Cellular Diagnostics : Basic Principles, Methods, and Clinical Applications of Flow Cytometry > ISBN13: 9783805585552

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Summary

This book is the updated English version of the 2006 German bestseller Zelluläre Diagnostik, a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.

Wallace Henry Coulter - a Short Biography
Foreword
Preface
Background and Methodological Principles
Cytometry - a Definitive History of the Early Days	p. 1
Concept Developments in Flow Cytometry	p. 29
Technical Background and Methodological Principles of Flow Cytometry	p. 53
Technical and Methodological Basics of Slide-Based Cytometry	p. 89
Selection and Combination of Fluorescent Dyes	p. 107
Fluorescence-Resonance Energy Transfer (FRET)	p. 141
Quality Control and Standardization	p. 159
Flow-Cytometric Cell Sorting	p. 178
Characterization and Phenotyping of Cells
Characterization of T-Lymphocytes	p. 190
T-Cell Receptor Repertoire Analysis by Flow Cytometry	p. 200
Characterization of B-Lymphocytes	p. 211
Characterization of Natural Killer Cells	p. 230
Lymphocyte Subsets in the Peripheral Blood of Healthy Children	p. 261
Quantitative Assessment of Plasmacytoid and Myeloid Dendritic Cells	p. 272
Peripheral Blood Stem Cells	p. 286
Endothelial Progenitor Cells	p. 305
Mesenchymal Progenitor Cells	p. 317
Analysis of Cell Functions
Determination of Cell Physiological Parameters: pH, Ca[superscript 2+], Glutathione, Transmembrane Potential	p. 325
Oxidative Burst, Phagocytosis and Expression of Adhesion Molecules	p. 343
Platelet Function and Thrombopoietic Activity	p. 377
DNA and Proliferation Measurements in Flow Cytometry	p. 390
Multidrug Resistance	p. 426
Flow-Cytometric Analysis of Intracellularly Stained Cytokines, Phosphoproteins, and Microbial Antigens	p. 459
Detection of Antigen-Specific T-Cells using MHC Multimers or Functional Parameters	p. 476
Diagnostic Indications
Immunophenotyping CD4 T-Cells to Monitor HIV Disease	p. 503
Primary Immunodeficiency Diseases	p. 524
Diagnostics for Common Variable Immunodeficiency Syndrome	p. 540
Monitoring of Organ-Transplanted Patients	p. 558
Flow-Cytometric Analysis of Cells from the Respiratory Tract: Bronchoalveolar Lavage Fluid and Induced Sputum	p. 580
Pathophysiology and Immune Monitoring of Sepsis	p. 600
Flow-Cytometric Immunophenotyping of Acute Leukemias	p. 612
Flow Cytometry in the Diagnosis of Non-Hodgkin's Lymphomas	p. 642
Paroxysmal Nocturnal Hemoglobinuria	p. 668
Fetal Erythrocytes	p. 680
Flow-Cytometric Cross-Match for the Determination of Platelet Antibodies	p. 687
Residual Cells in Blood Products	p. 694
Enrichment of Disseminated Tumor Cells	p. 714
Subject Index	p. 725
Table of Contents provided by Blackwell. All Rights Reserved.

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