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Preface | |
Single-Particle Imaging and Tracking | |
Three Dimensional Particle Tracking In A Laser Scanning Fluorescence Microscope | |
Introduction | |
Image-Based Single-Particle Tracking Methods | |
Advanced Fluorescence Microscopy Techniques for Single-Particle Tracking | |
Two-Photon Excitation Microscopy | |
3-D Tracking in Image-Based SPT Approaches | |
3-D Tracking in Laser Scanning Microscopes | |
Instrumentation | |
Background Noise | |
Simultaneous Two-Particle Tracking | |
Application: Chromatin Dynamics in Interphase Cells | |
Conclusions | |
The Tracking Of Individual Molecules In Cells And Tissues | |
Introduction | |
Single-Molecule and Single-Particle Localization | |
Positional Accuracy | |
Tracking | |
Trajectory Analysis | |
Applications | |
Conclusions | |
Messenger Rna Trafficking In Living Cells | |
Intranuclear Structure and Dynamics | |
FCS and FRAP Studies of Nuclear mRNP Mobility | |
Single-Particle Tracking of mRNA Molecules | |
Single-Particle Tracking of Specific, Native mRNPs | |
In Vivo Labeling of Native BR2 mRNPs | |
Outlook: Light Sheet-Based Single-Molecule Microscopy | |
Quantum Dots: Inorganic Fluorescent Probes For Single-Molecule Tracking Experiments In Live Cells | |
Introduction | |
Fluorescent Labels for Single-Molecule Tracking in Cells | |
Optical Properties of Colloidal Quantum Dots | |
Synthesis of Colloidal Fluorescent Quantum Dots | |
Surface Chemistry for the Water-Solubilization of Quantum Dots | |
Interfacing Quantum Dots with Biology | |
Single Quantum Dot Tracking Experiments in Live Cells | |
Conclusions and Perspectives | |
Energy Transfer on the Nanoscale | |
Single-Pair Fret: An Overview With Recent Applications And Future Perspectives | |
Introduction | |
Principles of FRET | |
spFRET in Solution | |
spFRET on Immobilized Molecules | |
Future Prospects | |
Alternating-Laser Excitation And Pulsed-Interleaved Excitation Of Single Molecules | |
Introduction | |
ALEX: The Principles of Operation | |
MyALEX | |
Nanosecond-ALEX/Pulsed Interleaved Excitation (PIE) | |
msALEX | |
Three-Color ALEX | |
Conclusions and Outlook | |
Unraveling The Dynamics Brdiging Protein Structure And Function One Molecule At A Time | |
Introduction | |
Converting Chemical Energy to Mechanical Work: Molecular Motors | |
Allostery in Proteins | |
Enzyme Catalysis | |
Conclusions | |
Quantitative Distance And Position Measurement Using Single-Molecule Fret | |
Introduction | |
Fundamentals of FRET | |
FRET as a Spectroscopic Ruler: Initial Experiments and Limitations | |
Measuring the Quantum Yield | |
The Orientation of Donor and Acceptor Molecules | |
Accurate FRET Measurements Using Fluorescence Correlation Spectroscopy | |
FRET-Based Triangulation and the Nanopositioning System | |
Conclusions and Outlook | |
Single Molecules in Nanosystems | |
Coherent And Incoherent Coupling Between A Single Dipolar Emitter And Its Nanoenvironment | |
Introduction | |
Systems | |
Coupling of Two Oscillating Dipoles | |
A Dipole Close to a Surface | |
A Single Molecule and a Single Nanoparticle | |
Modification of the Spontaneous Emission and Quantum Efficiency by Nanoantennae | |
Conclusions | |
Energy Transfer In Single Conjugated Polymer Chains | |
Introduction | |
Why Single Chain Spectroscopy? | |
Experimental Approach and Material Systems | |
Photophysics of Single Conjugated Polymer Chains | |
Energy Transfer in Single Chains | |
Influence of Initial Excitation Energy on Energy Transfer | |
Conclusions | |
Reactions At The Single-Molecule Level | |
Introduction | |
Biocatalysis at the Single-Molecule Level | |
Chemocatalysis at the Single-Molecule Level | |
Visualizing Single-Molecule Diffusion In Nanochannel Systems | |
Introduction | |
Correlation of Structural and Dynamic Properties Using TEM and SMT | |
Phase Mixture | |
Heterogeneous Dynamics of a Single Molecule | |
Oriented Single Molecules with Switchable Mobility in Long Unidimensional Nanochannels | |
High Localization Accuracy of Single Molecules Down to the Single Channel Limit | |
Probing Chemical Interactions in Silica Thin Films Using Fluorescence Correlation Spectroscopy (FCS) | |
Functionalized Mesoporous Silica Structures | |
Single-Molecule Studies of Mesoporous Silica Structures for Drug-Delivery Applications | |
Conclusions and Outlook | |
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