Single-Molecule Techniques: A Laboratory Manual

by Selvin, Paul R; Taekjip, Ha

• ISBN13: 9780879697754
• ISBN10: 087969775X
• Edition: 1st
• Format: Paperback
• Copyright: 2007-11-19
• Publisher: Cold Spring Harbor Laboratory Press

Summary

This laboratory manual is the first to take researchers who know nothing about single-molecule analyses to the point where they can successfully design and execute appropriate experiments. Techniques range from in vivo and in vitro fluorescent-based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. Illustrations.

Table of Contents

Preface
The New Era of Biology In Singulo	p. 1
Single-Molecule FRET with Total Internal Reflection Microscopy	p. 3
In Vitro and In Vivo FIONA and Other Acronyms for Watching Molecular Motors Walk	p. 37
Colocalization of Fluorescent Probes: Accurate and Precise Registration with Nanometer Resolution	p. 73
Alternating-Laser Excitation of Single Molecules	p. 85
Orientation and Rotational Motions of Single Molecules by Polarized Total Internal Reflection Fluorescence Microscopy	p. 121
Imaging Gene Expression in Living Cells at the Single-Molecule Level	p. 149
Single-Virus Tracking in Live Cells	p. 171
Ultrasensitive Imaging in Live Cells Using Fluorescent Quantum Dots	p. 193
Imaging Real-Time Gene Expression in Living Systems	p. 209
Single-Molecule Imaging of Stochastic Signaling Events in Living Cells	p. 239
Fluorescence Correlation Spectroscopy In Vitro and In Vivo	p. 259
Optical Traps to Study Properties of Molecular Motors	p. 279
High-Resolution Dual-Trap Optical Tweezers with Differential Detection	p. 297
Imaging and Nanomanipulation of an Actomyosin Motor	p. 325
Single-Molecule Studies Using Magnetic Traps	p. 347
Probing Polysaccharide and Protein Mechanics by Atomic Force Microscopy	p. 371
Single-Molecule Analysis of Nucleic Acids and DNA-Protein Interactions Using Nanopores	p. 395
Single-Molecule Gold-Nanoparticle Tracking with High Temporal and Spatial Resolution and without an Applied Load	p. 421
Advances in Surface-based Assays for Single Molecules	p. 431
Hydrodynamic Flow-stretching Assay for Single-Molecule Studies of Nucleic Acid-Protein Interactions	p. 461
Cautions	p. 493
Index	p. 501
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