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9780849300448

In Situ Hybridization in Electron Microscopy

by ;
  • ISBN13:

    9780849300448

  • ISBN10:

    0849300444

  • Format: Hardcover
  • Copyright: 2001-01-30
  • Publisher: CRC Press

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Supplemental Materials

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Summary

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods and describes the different stages in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products. The book includes principles of methodology and practical protocols, along with specific comments, in a two-column user-friendly format. With nearly 150 figures and tables, this book is an essential companion for any researcher in this field.

Table of Contents

General Introduction xi
Abbreviations xv
Probes
1(46)
Principles of Methodology
47(18)
Sample Preparation
65(22)
Post-Embedding Technique
87(64)
Pre-Embedding Technique
151(56)
Frozen Tissue Technique
207(46)
Semithin Sections
253(48)
Controls and Problems
301(20)
Examples of Results 321(52)
Appendices 373(56)
A - Equipement
379(16)
B - Reagents
395(34)
Glossary 429(18)
Index 447

Supplemental Materials

What is included with this book?

The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

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