In Situ Hybridization Protocols

by Darby, Ian A.

• ISBN13: 9780896036864
• ISBN10: 0896036863
• Edition: 2nd
• Format: Hardcover
• Copyright: 1999-09-01
• Publisher: Springer Verlag

Summary

RMIT Univ., Bundoora, Victoria, Australia. Presents state-of-the-art protocols of in situ hybridiza- tion. For cell or molecular biologists. Previous edition: c1994.

Table of Contents

Preface	v
Contributors	xi
PART 1 CHROMOSOMAL TECHNIQUES
Preparation of Human Partial Chromosome Paints from Somatic Cell Hybrids	3	(12)
Nicoletta Archidiacono
Rosalia Marzella
Cosma Spalluto
Margherita Pennacchia
Luigi Viggiano
Mariano Rocchi
DNA---Protein In Situ Covisualization for Chromosome Analysis	15	(14)
Henry H. Q. Heng
Barbara Spyropoulos
Peter B. Moens
Radioactive In Situ Hybridization to Animal Chromosomes	29	(22)
Graham C. Webb
Native Polytene Chromosomes of Drosophila melanogaster for Light and Electron Microscopic Observation of the Conformation and Distribution of Molecules	51	(18)
Ronald J. Hill
Margaret R. Mott
Released Chromatin or DNA Fiber Preparations for High-Resolution Fiber FISH	69	(14)
Henry H. Q. Heng
In Situ Hybridization to Polytene Chromosomes of Drosophila melanogaster and Other Dipteran Species	83	(20)
A. Marie Phillips
Jon Martin
Daniel G. Bedo
In Situ Hybridization to Polytene Chromosomes	103	(12)
Robert D. C. Saunders
Comparative Gene Mapping in Exotic Species Using FISH	115	(16)
Stephen A. Wilcox
Roland Toder
Pino Maccarone
Jennifer A. Marshall-Graves
PART 2 TISSUE TECHNIQUES
In Situ Hybridization with Nonradioactive Probes	131	(12)
Gwen V. Childs
Quantitiative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Heterocellular Systems	143	(14)
Catherine Le Moine
In Situ End-Labeling of Fragmented DNA and the Localization of Apoptosis	157	(8)
Tim D. Hewitson
Teresa Bisucci
Ian A. Darby
Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue Sections	165	(12)
Malcolm D. Smith
Angela Parker
Riyani Wikaningrum
Mark Coleman
In Situ Hybridization Protocols for Detection of Viral DNA Using Radioactive and Nonradioactive DNA Probes	177	(18)
Allison R. Jilbert
Signal Amplification for DNA and mRNA	195	(22)
Ernst J. M. Speel
Anton H. N. Hopman
Paul Komminoth
In Situ Localization of PCR-Amplified DNA and cDNA	217	(22)
Gerard J. Nuovo
Ultrastructural Detection of Nucleic Acids on Thin Sections of Tissue Embedded in Hydrophilic Resin	239	(20)
Dominique Le Guellec
In Situ Hybridization for Electron Microscopy	259	(20)
Ross F. Waller
Geoffrey I. McFadden
In Situ Hybridization of Whole-Mount Embryos	279	(12)
Murray Hargrave
Peter Koopman
cRNA Probes: Comparison of Isotopic and Nonisotopic Detection Methods	291	(16)
Teresa Bisucci
Tim D. Hewitson
Ian A. Darby
PART 3 APPLICATIONS
Molecular Cytogenetic Analysis of Sperm from Infertile Males Undergoing Intracytoplasmic Sperm Injection	307	(16)
William G. Kearns
Myung-Geol Pang
Darren Griffin
Lesil Brihn
Michael Stacey
Gustavo F. Doncel
Sergio Oehninger
Anabil A. Acosta
Stanton F. Hoegerman
Localization of HIV-1 DNA and Tumor Necrosis Factor-α mRNA in Human Brain Using Polymerase Chain Reaction In Situ Hybridization and Immunocytochemistry	323	(16)
Steven L. Wesselingh
Jonathan D. Glass
Index	339