Somatic Hybridization in Crop Improvement II

by Nagata, Toshiyuki; Bajaj, Y. P. S.

• ISBN13: 9783540411123
• ISBN10: 3540411127
• Format: Hardcover
• Copyright: 2002-02-01
• Publisher: Springer Nature

Summary

Somatic hybrids through the fusion of plant protoplasts have widened the genetic variability of cultivated plants. As "Somatic Hybridization in Crop Improvement I", published in 1994, this volume describes how this discipline can contribute to the improvement of crops. It comprises 24 chapters dealing with interspecific and intergeneric somatic hybridization and cybridization. It is divided into four sections:I. Cereals: Barley, rice, and wheat.II. Vegetables and Fruits: Arabidopsis, Asparagus, Brassica, chicory, Citrus, Cucumis, Diospyros, Ipomoea, and various Solanaceous species, e.g., tomato, potato, and eggplant.III. Medicinal and Aromatic Plants: Atropa, Dianthus, Nicotiana, and Senecio.IV. Legumes/Pasture Crops: Alfalfa.This book is tailored to the needs of advanced students, teachers and researchers in the fields of plant breeding, genetic engineering, and plant tissue culture.

Table of Contents

Section I Cereals
Somatic Hybridization Between Hordeum vulgare L. (Barley) and Daucus carota L. (Carrot)
H. Kisaka
M. Kisaka
A. Kanno
T. Kameya
Introduction	3	(1)
Protoplast Fusion and Culture of Fused Cells	4	(1)
Analysis of the Three Regenerated Plants	5	(2)
Characterization of Somatic Hybrids	7	(2)
Quantification and Effects of Betaine	9	(1)
Discussion	9	(3)
Protocol	12	(5)
References	14	(3)
Cybridization in Oryza sativa L. (Rice)
H. Akagi
Introduction	17	(2)
Somatic Hybridization	19	(8)
Charcteriztion of Rice Cybrids	27	(2)
Selection of CMS Cybrid Plants	29	(1)
Application of the Cybridization Method in Rice Breeding	29	(3)
Summary and Conclusion	32	(1)
Protocol	32	(5)
References	34	(3)
Somatic Hybridization Between Oryza sativa L. (Rice) and Hordeum vulgare L. (Barley)
H. Kisaka
M. Kisaka
A. Kanno
T. Kameya
Introduction	37	(1)
Protoplast Fusion and Culture	38	(2)
Analysis of Regenerated Plant	40	(1)
Assessment of Cold and Salt Tolerance	41	(2)
Protocol	43	(5)
References	46	(2)
Somatic Hybridization Between Triticum aestivum L. (Wheat) and Haynaldia villosa L.
G.M. Xia
A.F. Zhou
H.M. Chen
Introduction	48	(1)
Preparation and Treatment of Parental Protoplasts	49	(1)
Protoplast Fusion and Culture	50	(3)
Verfication of Hybridity	53	(6)
Growth and Development of Hybrid Plants	59	(2)
Summary and Conclusion	61	(1)
Protocol	62	(3)
References	64	(1)
Asymmetric Somatic Hybridization Between Triticum aestivum L. (Wheat) and Leymus chinensis (Tzin.) Tzvel
G.M. Xia
A.F. Zhou
F. Xiang
H.M. Chen
Introduction	65	(1)
Preparation and Inactivation of Wheat Protoplasts	65	(1)
Preparation of L. chinensis Protoplasts from Irradiated Calli	66	(3)
Fusion of Parental Protoplasts and Culture of Fusion Products	69	(1)
Identification of Hybrid Calli and Plants	70	(3)
Summary and Conclusion	73	(2)
Protocol	75	(6)
References	76	(5)
Section II Vegetables and Fruits
Somatic Hybridization Between Arabidopsis and Brassica
J. Siemens
M.D. Sacristan
Introduction	81	(1)
Somatic Hybridization	82	(8)
Summary and Conclusion	90	(1)
Protocol	91	(4)
References	92	(3)
Somatic Hybridization in Asparagus
H. Kunitake
M. Mii
Introduction	95	(2)
Somatic Hybridization	97	(8)
Summary and Conclusion	105	(3)
Protocol	108	(4)
References	110	(2)
Somatic Hybridization in Cichorium intybus L. (Chicory)
C. Rambaud
J. Vasseur
Introduction	112	(1)
Somatic Hybridization	113	(5)
Analysis of the Regenerated Plants	118	(2)
Summary and Conclusion	120	(1)
Protocol for the Regenaration of Somatic Hybrids	121	(3)
References	122	(2)
Cybridization in Citrus unshiu Marc. (Satsuma Mandarin) and C. sinensis (L.) Osbeck (Sweet Orange)
M. Yamamoto
S. Kobayashi
T. Yoshioka
R. Matsumoto
Introduction	124	(2)
Somatic Hybridization	126	(9)
Summary and Conclusion	135	(1)
Protocol	136	(3)
References	137	(2)
Somatic Hybridization in Cucumis
C.I. Jarl
Introduction	139	(3)
Somatic Hybridization	142	(6)
Summary and Conclusion	148	(1)
Protocol	148	(4)
References	149	(3)
Somatic Hybridization in Diospyros (Persimmon)
M. Tamura
R. Tao
Introduction	152	(1)
Somatic Hybridization	153	(6)
Summary and Conclusion	159	(1)
Protocol	160	(4)
References	162	(2)
Somatic Hybridization in Ipomoea (Sweet Potato) Species
M.M. Belarmino
T. Sasahara
Introduction	164	(2)
Protoplast Isolation	166	(3)
Protoplast Culture and Plant Regeneration	169	(3)
Interspecific Hybridization Between Sweet Potato and Wild Relatives	172	(5)
Asymmetric Protoplast Fusion Between Sweet Potato (Ipomoea batatas Lam.) and African Marigold (Tagetes erecta L.)	177	(3)
Summary and Conclusion	180	(3)
Protocol for Asymmetric Protoplast Fusion Between Sweet Potato (Ipomoea batatas Lam.) and Wild Relative, I. trifida Don	183	(5)
References	184	(4)
Somatic Hybridization Between Lycopersicon esculentum Mill. (Tomato) and Wild Nontuberous Solanum Species
T. Gavrilenko
Introduction	188	(1)
Methods of Protoplast Isolation, Protoplast Culture, and Plant Regeneration	189	(2)
Production and Analysis of Intergeneric Somatic Hybrids	191	(5)
Summary and Conclusion	196	(1)
Protocol	197	(2)
References	198	(1)
Somatic Hybridization Between Lycopersicon esculentum Mill. (Tomato) and Solanum melongena L. (Eggplant)
V.M. Samoylov
K.C. Sink
Introduction	199	(1)
Somatic Hybridization	200	(2)
Analysis of Somatic Hybrids	202	(5)
Elimination of the Donor Genome	207	(4)
Polyploidization and Elimination of the Donor Genome	211	(2)
Protocol	213	(4)
References	214	(3)
Somatic Hybridization Between Lycopersicon esculentum Mill. (Tomato) and Solanum ochranthum Dun.
J.R. Stommel
R.S. Kobayashi
S.L. Sinden
Introduction	217	(1)
Isolation of Protoplasts	218	(1)
Culture of Protoplasts	219	(2)
Fusion of Protoplasts and Regeneration of Somatic Hybrids	221	(1)
Regeneration and Characterization of Somatic Hybrids	222	(7)
Summary and Conclusion	229	(1)
Protocol	229	(4)
References	230	(3)
Somatic Hybridization Between Solanum melongena L. (Eggplant) and Solanum sanitwongsei Craib.
H. Asao
S. Arai
M. Hirai
Introduction	233	(1)
Somatic Hybridization	234	(7)
Summary and Conclusions	241	(2)
Protocol	243	(2)
References	243	(2)
Somatic Hybridization Between Solanum commersonii Dun. and S. tuberosum L. (Potato)
T. Cardi
Introduction	245	(1)
Somatic Hybridization	246	(12)
Summary and Conclusion	258	(1)
Protocol	259	(5)
References	260	(4)
Somatic Hybridization Between Solanum tuberosum L. (Potato) and Solanum phureja
S. Millam
P. Davie
Introduction	264	(2)
Somatic Hybridization	266	(7)
Summary and Conclusion	273	(4)
References	273	(4)
Section III Medicinal and Aromatic Plants
Somatic Hybridization in Dianthus Species
M. Nakano
Y. Hoshino
M. Mu
Introduction	277	(1)
Protoplast Isolation	278	(1)
Protoplast Culture	279	(1)
Protoplast Fusion	280	(1)
Regeneration of Somatic Hybrids	281	(8)
Summary and Conclusion	289	(1)
Protocol	290	(2)
References	290	(2)
Somatic Hybridization Between Nicotiana sylvestris Speg. & Comes and N. plumbaginifolia Viv.
C.C. Chen
Y.Y. Kao
F.M. Lee
R.F. Lin (With 4 Figures)
Introduction	292	(2)
Somatic Hybridization	294	(4)
Applications of Somatic Hybrids	298	(2)
Summary and Conclusion	300	(1)
Protocol	301	(3)
References	302	(2)
Somatic Hybridization Between Nicotiana tabacum L. (Tobacco) and Atropa belladonna L. (Deadly Nightshade)
M.K. Zubko
E.I. Zubko
O.A. Khvedynich
S.V. Lopato
S.A. Latipov
Yu. Yu. Gleba
Introduction	304	(4)
Somatic Hybridization	308	(12)
Conclusions and Prospects	320	(2)
Protocol	322	(6)
References	324	(4)
Somatic Hybridization and Cell Grafting in Senecio
G. Wang
H. Binding
Introduction	328	(1)
Results and Discussion	328	(7)
Summary and Conclusion	335	(1)
Protocol	335	(6)
References	336	(5)
Section IV Legumes/Pasture Crops
Somatic Hybridization Between Medicago sativa L. (Alfalfa) and Lotus corniculatus L. (Birdsfoot Trefoil)
M. Niizeki
Introduction	341	(1)
Symmetric Somatic Cell Hybridization	342	(3)
Asymmetric Somatic Cell Hybridization	345	(6)
Summary and Conclusion	351	(2)
Protocol	353	(3)
References	354	(2)
Somatic Hybridization Between Medicago sativa L. (Alfalfa) and Medicago falcata L.
S. Arcioni
F. Damiani
F. Paolocci
F. Pupilli
Introduction	356	(2)
Isolation and Fusion of Protoplasts	358	(2)
Culture and Selection of Fused Protoplasts	360	(2)
Regeneration of Somatic Hybrids	362	(1)
Indentification and Characterization of Somatic Hybrid Plants	363	(6)
Summary and Conclusion	369	(1)
Protocol	370	(5)
References	371	(4)
Subject Index	375