Super Resolution Optical Imaging and Microscopy Methods, Algorithms, and Applications

by Qu, Junle; Yang, Zhigang

• ISBN13: 9783527349869
• ISBN10: 3527349863
• eBook ISBN(s): 9783527835522
• Edition: 1st
• Format: Hardcover
• Copyright: 2023-12-18
• Publisher: Wiley-VCH
• More Book Details

Summary

Extremely comprehensive resource containing cutting-edge and practical knowledge of super-resolution optical imaging

This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward.

Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include:

• Optical techniques, fluorescent probe design, and algorithm development
• Recent highlight and breakthroughs in biology using SRM methods
• The overall success of SRM in biological inventions
• The future direction and scope of the field

This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.

Author Biography

Dr. Junle Qu is a Professor in the College of Physics and Optoelectronic Engineering at Shenzhen University and the director of Center for Biomedical Optics and Photonics of Shenzhen University. His current research interests include biomedical optical imaging and imaging guided optical therapy. He has published more than 500 papers in peer reviewed journals.

Dr. Zhigang Yang is currently an associate Professor in the College of Physics and Optoelectronic Engineering, Shenzhen University and a member of Center for Biomedical Optics and Photonics of Shenzhen University. His research interests mainly focus on development of optical probes and biological imaging, including super-resolution fluorescent imaging, fluorescence lifetime imaging, and Visible-Near Infrared I/II imaging.

Table of Contents

1 SUPER RESOLUTION MICROSCOPY (SRM): BRIEF INTRODUCTION

2 CLASSIFICATIONS OF SRM: SCANNING NEAR-FIELD OPTICAL MICROSCOPY AND FAR-FIELD OPTICAL SUPER-RESOLUTION MICROSCOPY
2.1 Scanning Near-field Optical Microscopy
2.2 Far-field Optical Super-Resolution Microscopy

3 POINT SPREAD FUNCTION ENGINEERING SUPER-RESOLUTION MICROSCOPY
3.1 STED
3.2 GSD
3.3 RESOLFT

4 SINGLE MOLECULE LOCALIZATION MICROSCOPY
4.1 STORM
4.2 PALM/FPALM
4.3 SOFI
4.4 PAINT
4.5 MINFLUX

5 FREQUENCY-DOMAIN-PROCESSING TECHNOLOGY

6 DEEP LEARNING BASED SR MICROSCOPY

7 FLUORESCENT MATERIALS FOR SRM
7.1 Fluorescent protein
7.2 Organic fluorescent probe
7.3 Fluorescent metal complex
7.4 Fluorescent nanoparticles/quantum dots/carbon nanotubes

8 LABEL-FREE SUPER-RESOLUTION IMAGING: EVADING THE FLUORESCENT PROBES

9 APPLICATIONS OF SUPER RESOLUTION MICROSCOPY

10 CONCLUSION AND FUTURE PERSPECTIVE: CRITICAL ASSESSMENT AND THE SCOPE OF ADVANCEMENT