Surface Plasmon Resonance

by de Mol, Nico J.; Fischer, Marcel J. E.

• ISBN13: 9781607616696
• ISBN10: 1607616696
• Edition: 1st
• Format: Hardcover
• Copyright: 2010-03-17
• Publisher: Humana Pr Inc

Summary

While commercial instruments have expanded the usage and the related literature has increased, the quality of surface plasmon resonance (SPR) research has been hindered by a lack of knowledge of the processes that influence the SPR signal. In Surface Plasmon Resonance: Methods and Protocols, experts in the field present a wide variety of applications involving commercially available SPR instruments. The heart of the SPR technique is to construct a dedicated surface for an assay. Protocols describe such surfaces for many needs, including the study of membrane bound proteins. Beyond the protocols-based chapters, the volume also highlights the backgrounds of vital issues in the use of SPR, including processes occurring within the hydrogel environment of sensors and on lipid membrane surfaces as well as the analysis of kinetic information. Written in the highly successful Methods in Molecular Biology'„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Surface Plasmon Resonance: Methods and Protocols fills a need for well-described, hands-on SPR experimental protocols and promises to inspire the adaptation of these techniques to fit the needs of labs around the world.

Table of Contents

Preface	p. v
Contributors	p. ix
Surface Plasmon Resonance: A General Introduction	p. 1
The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing	p. 15
Amine Coupling Through EDC/NHS: A Practical Approach	p. 55
High-Affinity Immobilization of Proteins Using Biotin- and GST-Based Coupling Strategies	p. 75
A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surface Plasmon Resonance	p. 91
Affinity Constants for Small Molecules from SPR Competition Experiments	p. 101
Surface Plasmon Resonance Signal Enhancement for Immunoassay of Small Molecules	p. 113
High-Throughput Kinase Assay Based on Surface Plasmon Resonance	p. 131
SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads	p. 147
Carbohydrate-Lectin Interactions Assayed by SPR	p. 157
DNA Sensors Based on Mixed Self-Assembled DNA/Alkanethiol Films	p. 179
Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors	p. 191
Capture of Intact Liposomes on Biacore Sensor Chips for Protein-Membrane Interaction Studies	p. 201
Surface Plasmon Resonance Spectroscopy for Studying the Membrane Binding of Antimicrobial Peptides	p. 213
Surface Plasmon Resonance Spectroscopy in Determination of the Interactions Between Amyloid ß Proteins (Aß) and Lipid Membranes	p. 225
Incorporation of a Transmembrane Protein into a Supported 3D-Matrix of Liposomes for SPR Studies	p. 237
Application of Surface Plasmon Resonance Spectroscopy to Study G-Protein Coupled Receptor Signalling	p. 249
Integration of SPR Biosensors with Mass Spectrometry (SPR-MS)	p. 261
SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry	p. 269
Subject Index	p. 283
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