Techniques in HIV Research

by Aldovini, Anna; Walker, Bruce D.

• ISBN13: 9780716770060
• ISBN10: 0716770067
• Format: Spiral Bound
• Copyright: 1993-09-23
• Publisher: Oxford University Press

Summary

Techniques in HIV Research is a comprehensive manual for the beginning and seasoned AIDS investigator alike. Designed to introduce researchers new to HIV investigation to the wide variety of available techniques and approaches, it presents a broad range of laboratory techniques for investigating the basic virology, immunology, and molecular biology of the AIDS virus. For the more experienced researcher, the manual offers the latest techniques available--in a format that highlights the most appropriate methodologies designed to circumvent the common problems and pitfalls of AIDS research. The first such manual in this field, Techniques in HIV Research is essential for the large number of academic, private, and clinical laboratories requiring an up-to-the-minute guide to the current technology in this fast-moving field.

Table of Contents

Viral Diagnostics

HIV Antibody Detection in Serum

Detection of HIV in Clinical Specimens

Basic Virologic Techniques

Virus Isolation and Production

Quantitative Assays for Virus Infectivity

Quantitative Assays for Virus Detection

Virus Purification, Preparation of Infectious Virus Stock, and Virus Storage

Proviral DNA Cloning, Julie Overbaugh

Transfection of Molecularly Cloned HIV Genomes

Virus-Specific Host Immune Response

Neutralizing Antibodies

Nonrestricted Forms of Anti-HIV-1 Cytoxicity

HIV-1-Specific Cytoxic T Lymphocytes

T Helper Cell Responses

Evaluation of Candidate Anti-Hiv Agents in Vitro

Evaluation of Candidate Anti-HIV agents in vitro

Table of Contents provided by Publisher. All Rights Reserved.