Definition	p. 1
Structure and expression of a eukaryote gene coding for an mRNA and a protein	p. 3
Parameters of description of a genome	p. 5
Sequencing of whole genomes	p. 9
Vectors and cloning	p. 13
Restriction enzymes	p. 15
Electrophoresis of nucleic acids	p. 19
Description of a plasmid and a phagemid	p. 21
Description of a bacteriophage and a cosmid	p. 23
Description of a YAC and other large vectors	p. 25
Molecular cloning	p. 27
Genetic transformation of bacteria and yeasts	p. 31
Labelling of nucleic acids and hybridization	p. 33
DNA labelling	p. 35
Molecular hybridization	p. 39
In situ hybridization of mRNA	p. 43
DNA libraries and screening	p. 47
Construction of a genomic DNA library	p. 49
Construction of a cDNA library	p. 51
Screening of a library	p. 53
Differential screening : subtractive libraries, AFLP-cDNA	p. 57
Differential display RT-PCR	p. 61
Differential screening by SSH : suppression subtractive hybridization	p. 65
Differential screening by RDA : representational difference analysis	p. 69
EST : expressed sequence tags	p. 73
DNA microarrays : DNA microchips, cDNA filters	p. 77
Characterization of a gene	p. 89
DNA sequencing	p. 91
PCR : polymerase chain reaction	p. 97
RACE : rapid amplification of cDNA ends	p. 99
Genome walking by PCR	p. 103
RT-PCR : reverse transcriptase PCR	p. 105
In vitro transcription	p. 109
Determination of transcription initiation site	p. 111
Functional analysis of promoters	p. 113
Gel retardation	p. 117
DNase I footprinting	p. 119
Genetic transformation of eukaryotes	p. 121
Genetic transformation of plants by Agrobacterium tumefaciens	p. 123
Direct gene transfer into plant protoplasts	p. 129
Direct gene transfer by biolistics	p. 131
Genetic transformation of animal cells	p. 133
Cloning of animals	p. 137
Transient expression	p. 141
Analysis of gene function	p. 143
Recombinant proteins	p. 145
Baculoviruses of insects, vectors of expression of foreign genes	p. 149
Yeast two hybrid system	p. 155
Site-directed mutagenesis	p. 161
Mutation complementation in yeast	p. 167
Knock-out of genes in yeast	p. 171
Gene tagging	p. 175
RNA interference	p. 181
Polymorphism of a genome	p. 185
Molecular markers	p. 187
Genetic and physical maps	p. 193
PFGE : pulse field gel electrophoresis	p. 197
RFLP : restriction fragment length polymorphism	p. 201
RAPD : random amplified polymorphic DNA	p. 203
AFLP : amplified fragment length polymorphism	p. 205
Retromarkers	p. 207
SSCP : single strand conformation polymorphism	p. 213
DGGE : denaturing gel gradient electrophoresis	p. 215
SNP : single nucleotide polymorphism	p. 217
Simple sequence repeats (SSR) : microsatellites	p. 219
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