Tetrahymena Thermophila

by Collins

• ISBN13: 9780123859679
• ISBN10: 0123859670
• Format: Hardcover
• Copyright: 2012-03-28
• Publisher: Elsevier Science

Summary

This new volume of Methods in Cell Biology looks at Tetrahymena thermophila, a model organism in experimental biology. Covering sections on Systems perspectives and Operating principles, chapters are written by experts in the field. With cutting edge material, this comprehensive collection is intended to guide researchers of Tetrahymena thermophila for years to come. Covers sections on Systems perspectives and Operating principles Chapters are written by experts in the field. Cutting edge material amking this a truely comprehensive collection.

Table of Contents

Contributors	p. xi
Preface	p. xiii
Introduction
Perspectives on the Ciliated Protozoan Tetrahymena thermophila
Introduction	p. 4
Historical Contributions	p. 4
Compelling Opportunities	p. 5
Chapter Logic	p. 6
Acknowledgments	p. 6
References	p. 6
Systems Perspectives
The Life and Times of Tetrahymena
Introduction	p. 12
Brief History	p. 12
Description	p. 13
Life Cycles and Breeding Systems	p. 22
Evolution of Tetrahymena	p. 23
Perspective	p. 24
References	p. 25
Nuclear Dualism
Vegetative Cell Division	p. 30
Sexual Reproduction	p. 31
Chromatin Structure	p. 32
Molecular Events of MAC Anlagen Development	p. 35
Nuclear Transport	p. 44
References	p. 45
Whole Genome Studies of Tetrahymena
Introduction	p. 54
To Make a Long Story Short: A Brief History of the Tetrahymena MAC Genome Project	p. 54
Examples of Use	p. 55
Near-Term Goals for Improvement of Genomic Resources	p. 59
Looking Further Ahead	p. 68
Concluding Remarks	p. 74
Acknowledgments	p. 75
References	p. 75
From Molecules to Morphology: Cellular Organization of Tetrahymena thermophila
Overview	p. 84
Basal Bodies	p. 86
Cilia	p. 90
Cortical Microtubular Arrays and Non-microtubular Fibers	p. 96
Epiplasm (Membrane Skeleton)	p. 99
Apical Crown, Apical Band, and Contractile Ring	p. 101
Oral Apparatus	p. 103
Cytoproct	p. 108
Contractile Vacuole	p. 109
Mitochondria	p. 110
Nuclear Structure and Nuclear-Cortical Interaction	p. 115
Identification of Genes Involved in Cortical Organization	p. 120
Acknowledgments	p. 123
References	p. 124
Conservation and Innovation in Tetrahymena Membrane Traffic: Proteins, Lipids, and Compartments
Introduction	p. 142
Recent Work on Membrane Traffic in Tetrahymena	p. 146
Studies on Membrane Lipids in Tetrahymena	p. 154
Conservation Versus Innovation	p. 161
Using Expression Data to Elucidate Pathways of Membrane Traffic	p. 165
Acknowledgments	p. 169
References	p. 169
Developmental progression of Tetrahymena through the cell cycle and conjugation
Introduction	p. 178
Checkpoints and Developmental Contingencies	p. 179
Vegetative Development (Asexual Reproduction by Cellular Fission)	p. 180
Alternatives to Cell Division	p. 198
Conjugal Development	p. 198
Developmental Disruptions	p. 204
Overview of the Developmental Logic of Conjugation	p. 225
Acknowledgments	p. 229
References	p. 229
Operating Principles
Tetrahymena in the Laboratory: Strain Resources, Methods for Culture, Maintenance, and Storage
Introduction	p. 240
Tetrahymena thermophila Strains	p. 240
Other Tetrahymena Species	p. 245
Cell Culture Media	p. 246
Cell Culture	p. 256
Long-Term Storage	p. 267
Acknowledgments	p. 270
References	p. 270
Natural Populations and Inbred Strains of Tetmhymena
Introduction/Background	p. 278
The Species Problem	p. 279
Tetrahymena Collections	p. 280
Species of Tetrahymena	p. 283
Methods for Collecting and Identification of Tetrahymena	p. 287
References	p. 298
Tetrahymena thermophila Genetics: Concepts and Applications
Introduction	p. 302
Fundamental Concepts of Tetrahymena Genetics	p. 302
Basic Genetic Operations	p. 308
Genetic Mapping	p. 315
Isolating and Using Mutants: Identifying the Mutant Gene	p. 318
Acknowledgments	p. 323
References	p. 323
Transformation and Strain Engineering of Tetrahymena
Introduction	p. 328
Strategies for Manipulating the Genome of Tetrahymena Strains	p. 329
Generating Gene Knockouts	p. 330
Engineering Strains for Expression of Tagged Alleles	p. 333
Selectable Markers and Expression Vectors	p. 336
The Use of Heterokaryon Strains for the Study of Essential Genes	p. 337
Executing Biolistics Transformation and Electroporation	p. 337
Summary	p. 343
Acknowledgments	p. 343
References	p. 344
Biochemical Approaches Including the Design and Use of Strains Expressing Epitope-Tagged Proteins
Introduction: General Considerations for Strain Construction	p. 348
Whole-Cell Protein and Nucleic Acid Isolation, Detection, and Quantification	p. 350
Cell Lysate Preparation and Affinity Purification	p. 352
Detection of Recovered Complexes	p. 353
Acknowledgments	p. 354
References	p. 354
Cytological Analysis of Tetrahymena thermophila
Introduction	p. 358
Rationale	p. 358
Light Microscopy	p. 359
Electron Microscopy	p. 368
Discussion	p. 375
Acknowledgments	p. 376
References	p. 376
Purification of Tetrahymena Cytoskeletal Proteins
Overview	p. 379
Purification of Actin and Actin-Binding Proteins	p. 380
Purification of Tubulin and Tubulin-Associated Proteins	p. 382
Purification of Proteins from the Cortical Cytoskeleton	p. 382
Challenges and Opportunities	p. 388
Acknowledgments	p. 389
References	p. 389
Behavioral Bioassays and Their Uses in Tetrahymena
Introduction	p. 393
Materials and Methods	p. 396
Discussion and Summary	p. 406
References	p. 407
Tetrahymena in the Classroom
Introduction/Rationale	p. 412
Materials and Methods	p. 414
Discussion	p. 427
Acknowledgments	p. 429
References	p. 429
Index	p. 431
Volumes in Series	p. 443
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