Tetrahymena Thermophila

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  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2012-03-01

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This new volume of Methods in Cell Biology looks at Tetrahymena thermophila, a model organism in experimental biology. Covering sections on Systems perspectives and Operating principles, chapters are written by experts in the field. With cutting edge material, this comprehensive collection is intended to guide researchers of Tetrahymena thermophila for years to come. Covers sections on Systems perspectives and Operating principles Chapters are written by experts in the field. Cutting edge material amking this a truely comprehensive collection.

Table of Contents

Contributorsp. xi
Prefacep. xiii
Perspectives on the Ciliated Protozoan Tetrahymena thermophila
Introductionp. 4
Historical Contributionsp. 4
Compelling Opportunitiesp. 5
Chapter Logicp. 6
Acknowledgmentsp. 6
Referencesp. 6
Systems Perspectives
The Life and Times of Tetrahymena
Introductionp. 12
Brief Historyp. 12
Descriptionp. 13
Life Cycles and Breeding Systemsp. 22
Evolution of Tetrahymenap. 23
Perspectivep. 24
Referencesp. 25
Nuclear Dualism
Vegetative Cell Divisionp. 30
Sexual Reproductionp. 31
Chromatin Structurep. 32
Molecular Events of MAC Anlagen Developmentp. 35
Nuclear Transportp. 44
Referencesp. 45
Whole Genome Studies of Tetrahymena
Introductionp. 54
To Make a Long Story Short: A Brief History of the Tetrahymena MAC Genome Projectp. 54
Examples of Usep. 55
Near-Term Goals for Improvement of Genomic Resourcesp. 59
Looking Further Aheadp. 68
Concluding Remarksp. 74
Acknowledgmentsp. 75
Referencesp. 75
From Molecules to Morphology: Cellular Organization of Tetrahymena thermophila
Overviewp. 84
Basal Bodiesp. 86
Ciliap. 90
Cortical Microtubular Arrays and Non-microtubular Fibersp. 96
Epiplasm (Membrane Skeleton)p. 99
Apical Crown, Apical Band, and Contractile Ringp. 101
Oral Apparatusp. 103
Cytoproctp. 108
Contractile Vacuolep. 109
Mitochondriap. 110
Nuclear Structure and Nuclear-Cortical Interactionp. 115
Identification of Genes Involved in Cortical Organizationp. 120
Acknowledgmentsp. 123
Referencesp. 124
Conservation and Innovation in Tetrahymena Membrane Traffic: Proteins, Lipids, and Compartments
Introductionp. 142
Recent Work on Membrane Traffic in Tetrahymenap. 146
Studies on Membrane Lipids in Tetrahymenap. 154
Conservation Versus Innovationp. 161
Using Expression Data to Elucidate Pathways of Membrane Trafficp. 165
Acknowledgmentsp. 169
Referencesp. 169
Developmental progression of Tetrahymena through the cell cycle and conjugation
Introductionp. 178
Checkpoints and Developmental Contingenciesp. 179
Vegetative Development (Asexual Reproduction by Cellular Fission)p. 180
Alternatives to Cell Divisionp. 198
Conjugal Developmentp. 198
Developmental Disruptionsp. 204
Overview of the Developmental Logic of Conjugationp. 225
Acknowledgmentsp. 229
Referencesp. 229
Operating Principles
Tetrahymena in the Laboratory: Strain Resources, Methods for Culture, Maintenance, and Storage
Introductionp. 240
Tetrahymena thermophila Strainsp. 240
Other Tetrahymena Speciesp. 245
Cell Culture Mediap. 246
Cell Culturep. 256
Long-Term Storagep. 267
Acknowledgmentsp. 270
Referencesp. 270
Natural Populations and Inbred Strains of Tetmhymena
Introduction/Backgroundp. 278
The Species Problemp. 279
Tetrahymena Collectionsp. 280
Species of Tetrahymenap. 283
Methods for Collecting and Identification of Tetrahymenap. 287
Referencesp. 298
Tetrahymena thermophila Genetics: Concepts and Applications
Introductionp. 302
Fundamental Concepts of Tetrahymena Geneticsp. 302
Basic Genetic Operationsp. 308
Genetic Mappingp. 315
Isolating and Using Mutants: Identifying the Mutant Genep. 318
Acknowledgmentsp. 323
Referencesp. 323
Transformation and Strain Engineering of Tetrahymena
Introductionp. 328
Strategies for Manipulating the Genome of Tetrahymena Strainsp. 329
Generating Gene Knockoutsp. 330
Engineering Strains for Expression of Tagged Allelesp. 333
Selectable Markers and Expression Vectorsp. 336
The Use of Heterokaryon Strains for the Study of Essential Genesp. 337
Executing Biolistics Transformation and Electroporationp. 337
Summaryp. 343
Acknowledgmentsp. 343
Referencesp. 344
Biochemical Approaches Including the Design and Use of Strains Expressing Epitope-Tagged Proteins
Introduction: General Considerations for Strain Constructionp. 348
Whole-Cell Protein and Nucleic Acid Isolation, Detection, and Quantificationp. 350
Cell Lysate Preparation and Affinity Purificationp. 352
Detection of Recovered Complexesp. 353
Acknowledgmentsp. 354
Referencesp. 354
Cytological Analysis of Tetrahymena thermophila
Introductionp. 358
Rationalep. 358
Light Microscopyp. 359
Electron Microscopyp. 368
Discussionp. 375
Acknowledgmentsp. 376
Referencesp. 376
Purification of Tetrahymena Cytoskeletal Proteins
Overviewp. 379
Purification of Actin and Actin-Binding Proteinsp. 380
Purification of Tubulin and Tubulin-Associated Proteinsp. 382
Purification of Proteins from the Cortical Cytoskeletonp. 382
Challenges and Opportunitiesp. 388
Acknowledgmentsp. 389
Referencesp. 389
Behavioral Bioassays and Their Uses in Tetrahymena
Introductionp. 393
Materials and Methodsp. 396
Discussion and Summaryp. 406
Referencesp. 407
Tetrahymena in the Classroom
Introduction/Rationalep. 412
Materials and Methodsp. 414
Discussionp. 427
Acknowledgmentsp. 429
Referencesp. 429
Indexp. 431
Volumes in Seriesp. 443
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