Transgenesis and Targeted Mutagenesis in Immunology

by Bluethmann, Horst; Ohasti, Pamela S.

• ISBN13: 9780121057602
• ISBN10: 0121057607
• Format: Hardcover
• Copyright: 1994-05-01
• Publisher: Academic Pr

Summary

Investigations into the field of immunology are rapidly expanding with the use of genetically altered mice at the embryonic stage. This breakthrough laboratory guide provides a complete study of transgenesis and targeted mutagenesis in laboratory mice that will be valued by researchers looking for fresh observations and interpretations when designing future experiments. Special Features Include: Contributions of two Nobel Prize winners Addresses the use of mouse models in studying the immune system Targets gene distribution in embryonic stem cells and their introduction into blastocyte mice models Analyzes the in vivo functional loss of embryonic cells A practical, useful guidebook for individual researchers, laboratories, and libraries

Table of Contents

Transgenic Mouse Models to Study VDJ Recombination

Lymphocyte Development and Function in T Cell Receptor and RAG-1 Mutant Mice

RAG-2-Deficient Blastocyst Complementation

Role of CD4 and CD8 in Determining Cell Fate in the Thymus

T Cell Development in CD4, CD8, and p561ck Gene-Targeted Mice

T Cell Tolerance in T Cell Receptor Transgenic 1pr/1pr Mice

ThymocyteSelection and Peripheral Tolerance Using the Lymphocytic Choriomeningitis Virus as a Model Antigen

Peripheral T Cell Tolerance: Distinct Levels and Multistep Mechanisms

Peripheral Tolerance and the CD4 + T Cells

Studies of Tolerance, Inflammation, and Autoimmunity in Transgenic Mice

Immunological Studies Utilizing Cytokine Transgenic Mice

Effect of MHC Class II Encoding Transgenes on Autoimmunity in Nonobese Diabetic Mice

Development and Function of the Immune System in Mice with Targeted Disruption of the Interleukin-2 Gene

Immune Responses in Interleukin 4-Deficient Mice

Manipulation of Transgene-Encoded Self-Antigens to Explore Mechanisms of B Cell Tolerance

Transgenic Mouse Model for Peripheral B Cell Tolerance

Immunobiology of MHC Class I-Deficient Mice

MHC Class II Mutant Mice

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